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Nonlinear magic: multiphoton microscopy in the biosciences
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Journal Article
Nonlinear magic: multiphoton microscopy in the biosciences
2003
Overview
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals. Coupled with transgenic mouse models of disease and 'smart' genetically encoded fluorescent indicators, its use is now increasing exponentially. Properly applied, it is capable of measuring calcium transients 500 μm deep in a mouse brain, or quantifying blood flow by imaging shadows of blood cells as they race through capillaries. With the multitude of possibilities afforded by variations of nonlinear optics and localized photochemistry, it is possible to image collagen fibrils directly within tissue through nonlinear scattering, or release caged compounds in sub-femtoliter volumes.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Biological Science Disciplines - instrumentation
/ Biological Science Disciplines - methods
/ Biological Science Disciplines - trends
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Calcium
/ Image Enhancement - instrumentation
/ Imaging, Three-Dimensional - instrumentation
/ Imaging, Three-Dimensional - methods
/ Imaging, Three-Dimensional - trends
/ Lasers
/ Microscopy, Confocal - instrumentation
/ Microscopy, Confocal - methods
/ Microscopy, Confocal - trends
/ Microscopy, Fluorescence, Multiphoton - instrumentation
/ Microscopy, Fluorescence, Multiphoton - methods
/ Microscopy, Fluorescence, Multiphoton - trends
/ Optics
