Journal Article

A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein

Chih Shen Chuang, Yi Fan Fang, Horn-Jiunn Sheen, Hong-Ren Jiang, Pao Wei Tseng, Yu Jui Fan, Chih Zong Deng

2019

Overview

In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found.

• Publisher: Springer Science and Business Media LLC,Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/1647/2196/2197 / 692/700 / 9/10 / 96/63 / Biosensing Techniques / Biosensing Techniques - instrumentation / Biosensing Techniques - methods / C-Reactive Protein / C-Reactive Protein - chemistry / Diffusion coefficient / Fluorescence / Humanities and Social Sciences / Immunoassay / Immunoassay - instrumentation / Immunoassay - methods / Immunologic Tests / Immunologic Tests - instrumentation / Immunologic Tests - methods / multidisciplinary / Nanoparticles / Nanoparticles - chemistry / Science / Science (multidisciplinary) / Smartphone / Smartphones / Spectrometry, Fluorescence / Spectrometry, Fluorescence - instrumentation / Spectrometry, Fluorescence - methods