Journal Article

A Case of Prototheca zopfii Genotype 1 Infection in a Dog (Canis lupus familiaris)

Keating, M. Kelly, Armién, Aníbal G., DeLeon-Carnes, Marlene, Riet-Correa, Franklin, Silveira, Caroline S., Cesar, Deborah, Luhers, Martín, Giannitti, Federico

2018

Overview

Protothecosis is a rare disease caused by environmental algae of the genus Prototheca . These are saprophytic, non-photosynthetic, aerobic, colorless algae that belong to the Chlorellaceae family. Seven different species have been described. Prototheca zopfii genotype 2 and P. wickerhamii are most commonly involved in pathogenic infections in humans and animals. The objective of this work is to describe, for the first time, a case of protothecosis caused by P. zopfii genotype 1 in a dog. The dog, a 4-year-old mix bred male, was presented to a veterinary clinic in Montevideo, Uruguay, with multiple skin nodules, one of which was excised by surgical biopsy. The sample was examined histologically and processed by PCR, DNA sequencing, and restriction fragments length polymorphisms for the detection and genotyping of P. zopfii . In addition, transmission electron microscopy and scanning electron microscopy were performed. Histology showed severe ulcerative granulomatous dermatitis and panniculitis with myriads of pleomorphic algae. Algal cells were 4–17 µm in size, with an amphophilic, 2–4-µm-thick wall frequently surrounded by a clear halo, contained flocculant material and a deeply basophilic nucleus, and internal septae with daughter cells (endospores) consistent with endosporulation. Ultrastructurally, algal cells/endospores at different stages of development were found within parasitophorous vacuoles in macrophages. Prototheca zopfii genotype 1 was identified by molecular testing, confirming the etiologic diagnosis of protothecosis.

• Publisher: Springer Netherlands,Springer,Springer Nature B.V
• Subject: Algae / Animals / Biomedical and Life Sciences / Biopsy / Canis lupus familiaris / Case Report / Dermatitis / Developmental stages / DNA sequencing / DNA, Algal - chemistry / DNA, Algal - genetics / Dog Diseases - diagnosis / Dog Diseases - microbiology / Dog Diseases - pathology / Dogs / Eukaryotic Microbiology / Genetic aspects / Genetic polymorphisms / Genotype / Genotype & phenotype / Genotyping / Health aspects / Histocytochemistry / Infection / Infection - diagnosis / Infection - microbiology / Infection - pathology / Infection - veterinary / Life Sciences / Lupus / Macrophages / Male / Medical Microbiology / Microbial Ecology / Microbiology / Microscopy / Microscopy, Electron, Scanning / Microscopy, Electron, Transmission / Nodules / Nuclei / Plant Sciences / Polymerase Chain Reaction / Polymorphism, Restriction Fragment Length / Prototheca - classification / Prototheca - genetics / Prototheca - isolation & purification / Prototheca zopfii / Protothecosis / Rare diseases / Scanning electron microscopy / Sequence Analysis, DNA / Skin / Skin - pathology / Transmission electron microscopy / Uruguay / Vacuoles