Asset Details
Do you wish to reserve the book?
Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different ∆6- and ∆5-desaturases co-expressed with LPCAT from the marine diatom Phaeodactylum tricornutum
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different ∆6- and ∆5-desaturases co-expressed with LPCAT from the marine diatom Phaeodactylum tricornutum
Do you wish to request the book?
Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different ∆6- and ∆5-desaturases co-expressed with LPCAT from the marine diatom Phaeodactylum tricornutum
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different ∆6- and ∆5-desaturases co-expressed with LPCAT from the marine diatom Phaeodactylum tricornutum
2024
Overview
Continuous research on obtaining an even more efficient production of very long-chain polyunsaturated fatty acids (VLC-PUFAs) in plants remains one of the main challenges of scientists working on plant lipids. Since crops are not able to produce these fatty acids due to the lack of necessary enzymes, genes encoding them must be introduced exogenously from native organisms producing VLC-PUFAs. In this study we reported, in tobacco leaves, the characterization of three distinct ∆
6
-desaturases from diatom
Phaeodactylum tricornutum
, fungi
Rhizopus stolonifer
and microalge
Osterococcus tauri
and two different ∆
5
-desaturases from
P. tricornutum
and single-celled saprotrophic eukaryotes
Thraustochytrium sp
. The
in planta
agroinfiltration of essential ∆
6
-desaturases, ∆
6
-elongases and ∆
5
-desaturases allowed for successful introduction of eicosapentaenoic acid (20:5
∆5,8,11,14,17
) biosynthesis pathway. However, despite the desired, targeted production of ω3-fatty acids we detected the presence of ω6-fatty acids, indicating and confirming previous results that all tested desaturases are not specifically restricted to neither ω3- nor ω6-pathway. Nevertheless, the additional co-expression of acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) from
Phaeodactylum tricornutum
boosted the proportion of ω3-fatty acids in newly synthesized fatty acid pools. For the most promising genes combinations the EPA content reached at maximum 1.4% of total lipid content and 4.5% of all fatty acids accumulated in the TAG pool. Our results for the first time describe the role of LPCAT enzyme and its effectiveness in alleviating a bottleneck called ‘substrate dichotomy’ for improving the transgenic production of VLC-PUFAs in plants.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
1-Acylglycerophosphocholine O-acyltransferase
/ 631/449
/ 631/45
/ Agricultural and Veterinary Sciences
/ Bioteknologi med applikationer på växter och djur
/ Fatty Acid Desaturases - genetics
/ Fatty Acid Desaturases - metabolism
/ Fatty Acids, Omega-3 - biosynthesis
/ Fatty Acids, Omega-3 - metabolism
/ Humanities and Social Sciences
/ Lantbruksvetenskap och veterinärmedicin
/ Lipids
/ Metabolic Engineering - methods
/ Plants, Genetically Modified - genetics
/ Science
