Journal Article

Dynamic changes in the expression of MicroRNA-31 during inflammatory bowel disease-associated neoplastic transformation

Jin, Zhe, Harris, Mary, David, Stefan, Olaru, Alexandru V., Abraham, John M., Meltzer, Stephen J., Livak, Ferenc, Bayless, Theodore M., Vazquez, Christine, Yang, Jian, Kwon, John, Dassopoulos, Themistocles, Mori, Yuriko, Cheng, Yulan, Harpaz, Noam, Agarwal, Rachana, Selaru, Florin M., Paun, Bogdan

2011

Overview

Patients with inflammatory bowel disease (IBD) are at increased risk of developing colorectal cancer. Aberrant microRNA (miR) expression has been linked to carcinogenesis; however, no reports document a relationship between IBD-related neoplasia (IBDN) and altered miR expression. In the current study we sought to identify specific miR dysregulation along the normal–inflammation–cancer axis.MethodsmiR microarrays and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) were used to detect dysregulated miRs. Receiver operating characteristic curve analysis was employed to test for potential usefulness of miR-31 as a disease marker of IBDNs. In silico prediction analysis, Western blot, and luciferase activity measurement were employed for target identification.ResultsSeveral dysregulated miRs were identified between chronically inflamed mucosae and dysplasia arising in IBD. MiR-31 expression increases in a stepwise fashion during progression from normal to IBD to IBDN and accurately discriminated IBDNs from normal or chronically inflamed tissues in IBD patients. Finally, we identified factor inhibiting hypoxia inducible factor 1 as a direct target of miR-31.ConclusionsOur study reveals specific miR dysregulation as chronic inflammation progresses to dysplasia. MiR-31 expression levels increase with disease progression and accurately discriminates between distinct pathological entities that coexist in IBD patients. The novel effect of miR-31 on regulating factor inhibiting hypoxia inducible factor 1 expression provides a new insight on the pathogenesis of IBDN. (Inflamm Bowel Dis 2011;)

• Publisher: Oxford University Press,Wiley Subscription Services, Inc., A Wiley Company
• Subject: Biomarkers, Tumor - genetics / Biomarkers, Tumor - metabolism / Blotting, Western / Carcinogenesis / Cell Transformation, Neoplastic - genetics / Colon - metabolism / Colon - pathology / Colorectal cancer / Colorectal Neoplasms - etiology / Colorectal Neoplasms - metabolism / Colorectal Neoplasms - pathology / Disease Progression / Dysplasia / factor inhibiting hypoxia inducible factor 1 / Female / Gene Expression Profiling / Humans / Hypoxia / IBD‐related neoplasia / Inflammation / Inflammatory bowel disease / Inflammatory bowel diseases / Inflammatory Bowel Diseases - complications / Inflammatory Bowel Diseases - genetics / Inflammatory Bowel Diseases - metabolism / Intestine / Luciferases - metabolism / Male / microRNA / MicroRNAs / MicroRNAs - genetics / Middle Aged / miRNA / Neoplasia / Oligonucleotide Array Sequence Analysis / Polymerase chain reaction / Reverse Transcriptase Polymerase Chain Reaction / RNA, Messenger - genetics / Transformation / Western blotting