Journal Article

C-terminal amino acids are essential for human heat shock protein 70 dimerization

Hadi, Tarik, Seigneuric, Renaud, Gobbo, Jessica, Marcion, Guillaume, Chavanne, Evelyne, Artur, Yves, Briand, Loïc, Neiers, Fabrice, Garrido, Carmen

2015

Overview

The human inducible heat shock protein 70 (hHsp70), which is involved in several major pathologies, including neurodegenerative disorders and cancer, is a key molecular chaperone and contributes to the proper protein folding and maintenance of a large number of protein structures. Despite its role in disease, the current structural knowledge of hHsp70 is almost exclusively based on its Escherichia coli homolog, DnaK, even though these two proteins only share ∼50 % amino acid identity. For the first time, we describe a complete heterologous production and purification strategy that allowed us to obtain a large amount of soluble, full-length, and non-tagged hHsp70. The protein displayed both an ATPase and a refolding activity when combined to the human Hsp40. Multi-angle light scattering and bio-layer interferometry analyses demonstrated the ability of hHsp70 to homodimerize. The role of the C-terminal part of hHsp70 was identified and confirmed by a study of a truncated version of hHsp70 that could neither dimerize nor present refolding activity.

• Publisher: Springer,Springer Netherlands,Springer Nature B.V,Springer Verlag,Cell Stress Society International
• Subject: Adenosine triphosphatases / Amino acids / Biochemistry / Biochemistry, biophysics & molecular biology / Biochimie, biophysique & biologie moléculaire / Biomedical and Life Sciences / Biomedicine / Cancer / Cancer Research / Cell Biology / Chromatography / Circular Dichroism / Dimerization / Dimers / EC 3.6.1.- (dnaK protein, E coli) / Escherichia coli - metabolism / Escherichia coli Proteins / Escherichia coli Proteins - chemistry / Escherichia coli Proteins - metabolism / Escherichia coli Proteins/chemistry/metabolism / Fluorescence / Heat shock proteins / HSP70 Heat-Shock Proteins / HSP70 Heat-Shock Proteins - chemistry / HSP70 Heat-Shock Proteins - genetics / HSP70 Heat-Shock Proteins - metabolism / HSP70 Heat-Shock Proteins/chemistry/genetics/metabolism / Human health and pathology / Humans / Immunology / Isoelectric Point / Life Sciences / Molecular chaperones / Monomers / Neurosciences / Original Paper / Protein Refolding / Protein Structure, Secondary / Proteins / Recombinant Proteins / Recombinant Proteins - biosynthesis / Recombinant Proteins - chemistry / Recombinant Proteins - isolation & purification / Recombinant Proteins/biosynthesis/chemistry/isolation & purification / Sciences du vivant / Spectrometry, Fluorescence