Journal Article

Mesothelin, Calretinin, and Megakaryocyte Potentiating Factor as Biomarkers of Malignant Pleural Mesothelioma

Pérez-Padilla, José Rogelio, Rihs, Hans-Peter, Taeger, Dirk, Burek, Katarzyna, Casjens, Swaantje, Acosta-Saavedra, Leonor Concepción, Cabello-López, Alejandro, García-Bazan, Erik Marco, Del Razo, Luz M., Brüning, Thomas, Juárez-Pérez, Cuauhtémoc Arturo, Calderón-Aranda, Emma S., Jiménez-Ramírez, Carmina, Raiko, Irina, Pesch, Beate, Weber, Daniel Gilbert, Aguilar-Madrid, Guadalupe, Johnen, Georg, Ochoa-Vázquez, María Dolores, Torre-Bouscoulet, Luis

2019

Overview

Purpose Malignant pleural mesothelioma (MPM) is a highly lethal cancer caused by exposure to asbestos. Currently, the diagnosis is a challenge, carried out by means of invasive methods of limited sensitivity. This is a case–control study to evaluate the individual and combined performance of minimally invasive biomarkers for the diagnosis of MPM. Method A study of 166 incident cases of MPM and 378 population controls of Mestizo-Mexican ethnicity was conducted. Mesothelin, calretinin, and megakaryocyte potentiating factor (MPF) were quantified in plasma by ELISA. The samples were collected from 2011 to 2016. Results Based on ROC analysis and a preset specificity of 95%, the combination of the three biomarkers reached an AUC of 0.944 and a sensitivity of 82% in men. In women, an AUC of 0.937 and a sensitivity of 87% were reached. In nonconditional logistic regression models, the adjusted ORs in men were 7.92 (95% CI 3.02–20.78) for mesothelin, 20.44 (95% CI 8.90–46.94) for calretinin, and 4.37 (95% CI 1.60–11.94) for MPF. The ORs for women were 28.89 (95% CI 7.32–113.99), 17.89 (95% CI 3.93–81.49), and 2.77 (95% CI 0.47–16.21), respectively. Conclusions To our knowledge, this is the first study evaluating a combination of mesothelin, calretinin, and MPF, and demonstrating a sex effect for calretinin. The biomarker panel showed a good performance in a Mestizo-Mexican population, with high sensitivity and specificity for the diagnosis of MPM.

• Publisher: Springer US,Springer,Springer Nature B.V
• Subject: Age / Aged / Asbestos / Biomarkers / Biomarkers, Tumor - blood / Bone marrow cells / Calbindin 2 - blood / Calretinin / Diagnosis / Enzyme-Linked Immunosorbent Assay / Female / GPI-Linked Proteins - blood / Humans / Incidence / Internet resources / Invasiveness / Lung Neoplasms - blood / Lung Neoplasms - diagnosis / Lung Neoplasms - epidemiology / Male / Medical diagnosis / Medicine / Medicine & Public Health / Men / Mesothelioma / Mesothelioma - blood / Mesothelioma - diagnosis / Mesothelioma - epidemiology / Mesothelioma, Malignant / Mexico - epidemiology / Middle Aged / Minority & ethnic groups / Pleural Disease / Pleural Neoplasms - blood / Pleural Neoplasms - diagnosis / Pleural Neoplasms - epidemiology / Pneumology/Respiratory System / Population control / Predictive Value of Tests / Prognosis / Regression analysis / Regression models / Risk Assessment / Risk Factors / Sensitivity / Sensitivity analysis / Sex Factors / Women