Journal Article

A positron emission tomography imaging study to confirm target engagement in the lungs of patients with idiopathic pulmonary fibrosis following a single dose of a novel inhaled αvβ6 integrin inhibitor

Fahy, William A., Rizzo, Gaia, Searle, Graham E., Marshall, Richard P., Kang’ombe, Arthur R., Simpson, Juliet K., Fairman, David, Wilson, Frederick J., Kenny, Julia, Saleem, Azeem, Parry, Simon, Slack, Robert J., Porter, Joanna C., Molyneaux, Philip L., Paul, Lyn, Maher, Toby M., Saunders, Peter, Eames, Rhena, Siederer, Sarah, Vahdati-Bolouri, Mitra, Lukey, Pauline T., Chan, Robert, Cui, Yi, Sahota, Jagdeep

2020

Overview

Background Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive lung disease with poor prognosis and a significant unmet medical need. This study evaluated the safety, pharmacokinetics (PK) and target engagement in the lungs, of GSK3008348, a novel inhaled alpha-v beta-6 (αvβ6) integrin inhibitor, in participants with IPF. Methods This was a phase 1b, randomised, double-blind (sponsor unblind) study, conducted in the UK (two clinical sites, one imaging unit) between June 2017 and July 2018 (NCT03069989). Participants with a definite or probable diagnosis of IPF received a single nebulised dose of 1000 mcg GSK3008348 or placebo (ratio 5:2) in two dosing periods. In period 1, safety and PK assessments were performed up to 24 h post-dose; in period 2, after a 7-day to 28-day washout, participants underwent a total of three positron emission tomography (PET) scans: baseline, Day 1 (~ 30 min post-dosing) and Day 2 (~ 24 h post-dosing), using a radiolabelled αvβ6-specific ligand, [ 18 F]FB-A20FMDV2. The primary endpoint was whole lung volume of distribution (V T ), not corrected for air volume, at ~ 30 min post-dose compared with pre-dose. The study success criterion, determined using Bayesian analysis, was a posterior probability (true % reduction in V T  > 0%) of ≥80%. Results Eight participants with IPF were enrolled and seven completed the study. Adjusted posterior median reduction in uncorrected V T at ~ 30 min after GSK3008348 inhalation was 20% (95% CrI: − 9 to 42%). The posterior probability that the true % reduction in V T  > 0% was 93%. GSK3008348 was well tolerated with no reports of serious adverse events or clinically significant abnormalities that were attributable to study treatment. PK was successfully characterised showing rapid absorption followed by a multiphasic elimination. Conclusions This study demonstrated engagement of the αvβ6 integrin target in the lung following nebulised dosing with GSK3008348 to participants with IPF. To the best of our knowledge this is the first time a target-specific PET radioligand has been used to assess target engagement in the lung, not least for an inhaled drug. Trial registration clinicaltrials.gov: NCT03069989 ; date of registration: 3 March 2017.

• Publisher: BioMed Central,Nature Publishing Group,BMC
• Subject: Abnormalities / Administration, Inhalation / Aged / Alpha-v beta-6 / Antigens, Neoplasm / Bayes Theorem / Bayesian analysis / Butyrates - administration & dosage / Butyrates - pharmacokinetics / Butyrates - therapeutic use / Clinical trials / Conditional probability / Dosage / Double-Blind Method / Emission analysis / Endpoint Determination / Female / Fibrosis / Humans / Idiopathic pulmonary fibrosis / Idiopathic Pulmonary Fibrosis - diagnostic imaging / Idiopathic Pulmonary Fibrosis - drug therapy / Inhalation / Inhibitors / Integrin / Integrins - antagonists & inhibitors / IPF, positron emission tomography / Ligands / Lung diseases / Lungs / Male / Medical imaging / Medical prognosis / Medicine / Medicine & Public Health / Naphthyridines - administration & dosage / Naphthyridines - pharmacokinetics / Naphthyridines - therapeutic use / Nebulizers and Vaporizers / PET / Pharmacokinetics / Pneumology/Respiratory System / Positron emission / Positron emission tomography / Pulmonary fibrosis / Pyrazoles - administration & dosage / Pyrazoles - pharmacokinetics / Pyrazoles - therapeutic use / Pyrrolidines - administration & dosage / Pyrrolidines - pharmacokinetics / Pyrrolidines - therapeutic use / Reduction / Respiration / Safety / Tidal Volume - drug effects / Tomography / Treatment Outcome / αvβ6