Asset Details
Do you wish to reserve the book?
Wolbachia wAlbB inhibit dengue and Zika infection in the mosquito Aedes aegypti with an Australian background
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Wolbachia wAlbB inhibit dengue and Zika infection in the mosquito Aedes aegypti with an Australian background
Do you wish to request the book?
Wolbachia wAlbB inhibit dengue and Zika infection in the mosquito Aedes aegypti with an Australian background
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Wolbachia wAlbB inhibit dengue and Zika infection in the mosquito Aedes aegypti with an Australian background
2022
Overview
Biological control of mosquito vectors using the endosymbiotic bacteria
Wolbachia
is an emerging strategy for the management of human arboviral diseases. We recently described the development of a strain of
Aedes aegypti
infected with the
Wolbachia
strain
w
AlbB (referred to as the
w
AlbB2-F4 strain) through simple backcrossing of wild type Australian mosquitoes with a
w
AlbB infected
Ae
.
aegypti
strain from the USA. Field releases of male
w
AlbB2-F4 mosquitoes resulted in the successful suppression of wild populations of mosquitoes in the trial sites by exploiting the strain’s
Wolbachia-
induced cytoplasmic incompatibility. We now demonstrate that the strain is resistant to infection by dengue and Zika viruses and is genetically similar to endemic Queensland populations. There was a fourfold reduction in the proportion of
w
AlbB2-F4 mosquitoes that became infected following a blood meal containing dengue 2 virus (16.7%) compared to wild type mosquitoes (69.2%) and a 6–7 fold reduction in the proportion of
w
AlbB2-F4 mosquitoes producing virus in saliva following a blood meal containing an epidemic strain of Zika virus (8.7% in comparison to 58.3% in wild type mosquitoes). Restriction-site Associated DNA (RAD) sequencing revealed that
w
AlbB2-F4 mosquitoes have > 98% Australian ancestry, confirming the successful introduction of the
w
AlbB2 infection into the Australian genomic background through backcrossing. Genotypic and phenotypic analyses showed the
w
AlbB2-F4 strain retains the insecticide susceptible phenotype and genotype of native Australian mosquitoes. We demonstrate that the
Wolbachia w
AlbB2-F4, in addition to being suitable for population suppression programs, can also be effective in population replacement programs given its inhibition of virus infection in mosquitoes. The ease at which a target mosquito population can be transfected with
w
AlbB2, while retaining the genotypes and phenotypes of the target population, shows the utility of this strain for controlling the
Ae
.
aegypti
mosquitoes and the pathogens they transmit.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
