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Plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli and Klebsiella pneumoniae isolated from market retail fruits in Guangzhou, China
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Plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli and Klebsiella pneumoniae isolated from market retail fruits in Guangzhou, China
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Journal Article
Plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli and Klebsiella pneumoniae isolated from market retail fruits in Guangzhou, China
2019
Overview
As a result of the growing prevalence of the plasmid-mediated mobile colistin resistance gene
among Gram-negative bacteria, the surveillance of
has been globally applied. In our study, we aimed to shed light on the possibility of transmission of
-resistant isolates through market retail fruits.
Herein, 133 different fruit surface samples were collected and screened for the different MCR variants (
to
) using PCR and confirmed with sequencing. We identify for the first time
-carrying
and
from market retail fruits in Guangzhou, China. Minimum inhibitory concentrations were detected by the broth microdilution method. Liquid mating was performed to check the transferability of the
gene. Pulsed field gel electrophoresis analysis of S1 nuclease-digested DNA and Southern blotting were performed to check the location of the
gene. Then, whole-genome sequencing and in silico multilocus sequence typing analysis were performed.
We showed that
GB110 can mediate the spreading of antibiotic resistance genes through the food chain, while
GB015 was considered to be the progenitor of the most successful multidrug-resistant clone. Since fruits are usually consumed fresh, this may serve as a direct source of
-producing bacteria in humans that requires prompt surveillance and intervention to limit the spread of resistance.
