Journal Article

Direct micro-electric stimulation alters phenanthrene-degrading metabolic activities of Pseudomonas sp. strain DGYH-12 in modified bioelectrochemical system

Zhao, Jianfang, Wan, Guilong, Li, Xiaoguang, Sha, Beibei, Zhang, Xiaoxia, Wang, Xingbiao, Shi, Liuyang, Gao, Xiaolong, Huang, Zhiyong

2019

Overview

Bioelectrochemical systems (BESs) have great potential for treating wastewater containing polycyclic aromatic hydrocarbons (PAHs); however, detailed data on cell physiological activities in PAH biodegradation pathways stimulated by BESs are still lacking. In this paper, a novel BES device was assembled to promote the growth of Pseudomonas sp. DGYH-12 in phenanthrene (PHE) degradation. The results showed that in the micro-electric field (0.2 V), cell growth rate and PHE degradation efficiency were 22% and 27.2% higher than biological control without electric stimulation (BC), respectively. The extracellular polymeric substance (EPS) concentration in BES (39.38 mg L −1 ) was higher than control (33.36 mg L −1 ); moreover, the membrane permeability and ATPase activities were also enhanced and there existing phthalic acid and salicylic acid metabolic pathways in the strain. The degradation genes nahAc , pcaH , and xylE expression levels were upregulated by micro-electric stimulation. This is the first study to analyze the physiological and metabolic effect of micro-electric stimulation on a PHE-degrading strain in detail and systematically.

• Publisher: Springer Berlin Heidelberg,Springer Nature B.V
• Subject: Adenosine triphosphatase / adenosinetriphosphatase / Aquatic Pollution / biochemical pathways / Biodegradation / Biodegradation, Environmental / bioelectrochemistry / Biological control / cell growth / Earth and Environmental Science / Ecotoxicology / Electric fields / Electric Stimulation / Environment / Environmental Chemistry / Environmental Health / Environmental science / enzyme activity / Extracellular Polymeric Substance Matrix / Gene expression / gene expression regulation / genes / Growth rate / Membrane permeability / Metabolic Networks and Pathways / Metabolic pathways / Metabolism / Phenanthrene / phenanthrenes / Phenanthrenes - metabolism / Phthalic acid / Physiological effects / Physiology / Polycyclic aromatic hydrocarbons / Polycyclic Aromatic Hydrocarbons - metabolism / polymers / Pseudomonas / Pseudomonas - metabolism / Research Article / Salicylic acid / Stimulation / Waste Disposal, Fluid - methods / Waste Water / Waste Water Technology / Wastewater treatment / Water Management / Water Pollution Control