Journal Article

Preparation and Biochemical Activity of Copper-Coated Cellulose Nonwoven Fabric via Magnetron Sputtering and Alginate-Calcium Ion Complexation

Juszczak, Michał, Świerczyńska, Małgorzata, Woźniak, Katarzyna, Mrozińska, Zdzisława, Kudzin, Marcin H.

2024

Overview

Alginate-based materials have gained significant recognition in the medical industry due to their favorable biochemical properties. As a continuation of our previous studies, we have introduced a new composite consisting of cellulose nonwoven fabric charged with a metallic copper core (CNW-Cu0) covered with a calcium alginate (ALG−Ca2+) layer. The preparation process for these materials involved three main steps: coating the cellulose nonwoven fabric with copper via magnetron sputtering (CNW → CNW-Cu0), subsequent deposition with sodium alginate (CNW-Cu0 → CNW-Cu0/ALG−Na+), followed by cross-linking the alginate chains with calcium ions (CNW-Cu0/ALG−Na+ → CNW-Cu0/ALG−Ca2+). The primary objective of the work was to supply these composites with such biological attributes as antibacterial and hemostatic activity. Namely, equipping the antibacterial materials (copper action on representative Gram-positive and Gram-negative bacteria and fungal strains) with induction of blood plasma clotting processes (activated partial thromboplastin time (aPTT) and prothrombin time (PT)). We determined the effect of CNW-Cu0/ALG−Ca2+ materials on the viability of Peripheral blood mononuclear (PBM) cells. Moreover, we studied the interactions of CNW-Cu0/ALG−Ca2+ materials with DNA using the relaxation plasmid assay. However, results showed CNW-Cu0/ALG−Ca2+’s cytotoxic properties against PBM cells in a time-dependent manner. Furthermore, the CNW-Cu0/ALG−Ca2+ composite exhibited the potential to interact directly with DNA. The results demonstrated that the CNW-Cu0/ALG−Ca2+ composites synthesized show promising potential for wound dressing applications.

• Publisher: MDPI AG,MDPI
• Subject: Alginates - chemistry / Alginates - pharmacology / Alginic acid / Anti-Bacterial Agents - chemistry / Anti-Bacterial Agents - pharmacology / Antibacterial materials / Antibiotics / antimicrobial properties / Bacteria / Biocompatibility / Biological activity / Biomedical materials / Blood / Blood Coagulation - drug effects / Blood plasma / Calcium / Calcium (blood) / Calcium - chemistry / Calcium - metabolism / Calcium alginate / Calcium compounds / Calcium ions / Cell Survival - drug effects / Cellulose / Cellulose - chemistry / Cellulose fibers / Chemical properties / Chloride / Clotting / Comparative analysis / Composite materials / Copper / Copper - chemistry / Crosslinking / Cytotoxicity / Deoxyribonucleic acid / Design and construction / DNA / Fabrics / Gram-negative bacteria / Gram-positive bacteria / Hemostatics - chemistry / Hemostatics - pharmacology / Humans / Leukocytes, Mononuclear - drug effects / Magnetic properties / Magnetron sputtering / Medical materials / Microscopy / Nonwoven fabrics / Partial Thromboplastin Time / Peripheral blood / Physiological aspects / Prothrombin / Prothrombin Time / Seaweed meal / Sodium / Sodium alginate / Sputtering (Physics) / Testing / Thromboplastin / Time dependence / Wound healing