Journal Article

High-quality genome sequence of white lupin provides insight into soil exploration and seed quality

Bergès, Hélène, Hufnagel, Bárbara, Blein, Thomas, Laguerre, Malika, Marande, William, Péret, Benjamin, Schubert, Veit, Doumas, Patrick, Huneau, Cécile, Gouzy, Jérôme, Geu-Flores, Fernando, Crespi, Martin, Sallet, Erika, Carrere, Sébastien, Keller, Jean, Arribat, Sandrine, Aimé, Delphine, Marquès, Laurence, Guyot, Romain, Soriano, Alexandre, Taylor, Jemma, Salse, Jérôme, Mancinotti, Davide, Gallardo, Karine, Divol, Fanchon, Nelson, Matthew, Marques, André, Delaux, Pierre-Marc

2020

Overview

White lupin ( Lupinus albus L .) is an annual crop cultivated for its protein-rich seeds. It is adapted to poor soils due to the production of cluster roots, which are made of dozens of determinate lateral roots that drastically improve soil exploration and nutrient acquisition (mostly phosphate). Using long-read sequencing technologies, we provide a high-quality genome sequence of a cultivated accession of white lupin (2n = 50, 451 Mb), as well as de novo assemblies of a landrace and a wild relative. We describe a modern accession displaying increased soil exploration capacity through early establishment of lateral and cluster roots. We also show how seed quality may have been impacted by domestication in term of protein profiles and alkaloid content. The availability of a high-quality genome assembly together with companion genomic and transcriptomic resources will enable the development of modern breeding strategies to increase and stabilize white lupin yield. White lupin is an annual crop cultivated for protein rich seeds and can produce cluster roots for efficient phosphate acquisition. Here, the authors generate high quality genome assemblies of a cultivated accession, a landrace, and a wild relative and provides insight into soil exploration and seed quality.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 45 / 45/23 / 631/449/2491 / 631/449/2492 / 631/61/447/8 / Alkaloids - chemistry / Alkaloids - metabolism / Assemblies / Centromere - genetics / Clusters / Domestication / Ecotype / Evolution, Molecular / Exploration / Gene Dosage / Gene Duplication / Gene sequencing / Genetic Variation / Genome, Plant / Genomes / Genomic Structural Variation / Humanities and Social Sciences / Life Sciences / Lupinus - genetics / Lupinus - growth & development / Models, Genetic / Molecular Sequence Annotation / multidisciplinary / Nucleotide sequence / Plant Leaves - metabolism / Plant Proteins - metabolism / Plant Roots - genetics / Plant Roots - growth & development / Polymorphism, Single Nucleotide - genetics / Proteins / Quality / Repetitive Sequences, Nucleic Acid - genetics / Roots / Science / Science (multidisciplinary) / Seeds / Seeds - physiology / Sequence Analysis, DNA / Soil / Soil improvement / Soils / Synteny - genetics / Transcriptome - genetics / Vegetal Biology