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20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway
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20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway
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20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway
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Journal Article
20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway
2016
Overview
Aim： Our preliminary study shows that a bibenzyl compound isolated from Gastrodia elata, 2-[4-hydroxy-3-（4-hydroxybenzyl）benzyl]-4-（4-hydroxybenzyl）phenol （designated 20C）, protects PC12 cells against H202-induced injury. In this study we investigated whether 20C exerted neuroprotective action in a cell model of Parkinson＇s disease. Methods： A cell model of Parkinson＇s disease was established in PC12 cells by exposure to rotenone （4 pmol/L） for 48 h. Cell viability and apoptosis were assessed, and intracellular ROS level and the mitochondrial membrane potential （MMP） were detected. The expression of apoptosis-related proteins Bax, Bcl-2, cytochrome c, cleaved caspase-3, and oxidative stress-related proteins Nrf2, HO-1 and NQ01 were examined using Western blotting. The mRNA levels of HO-1 and NQ01 were determined with RT-PCR. The nuclear translocation of Nrf2 was observed with immunofluorescence staining. Results： Treatment with rotenone significantly increased the number of apoptotic cells, accompanied by marked increases in the Bax/ Bcl-2 ratio, cytochrome c release and caspase-3 activation. Rotenone also increased ROS accumulation, reduced MMP, and increased the nuclear translocation of Nrf2 as well as the mRNA and protein levels of the Nrf2 downstream target genes HO-1 and NQ01 in PC12 cells. Co-treatment with 20C （0.01-1 pmol/L） dose-dependently attenuated rotenone-induced apoptosis and oxidative stress in PC12 cells. Nrf2 knockdown by siRNA partially reversed the protective effects of 20C in rotenone-treated PC12 cells. Conclusion： The bibenzyl compound 20C protects PC12 cells from rotenone-induced apoptosis, at least in part, via activation of the Nrf2/ARE/HO-1 signaling pathway.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Antioxidant Response Elements - drug effects
/ Biomedical and Life Sciences
/ Heme Oxygenase-1 - metabolism
/ Neuroprotective Agents - chemistry
/ Neuroprotective Agents - pharmacology
/ NF-E2-Related Factor 2 - genetics
/ NF-E2-Related Factor 2 - metabolism
/ Original
/ Oxidative Stress - drug effects
/ Parkinson Disease, Secondary - drug therapy
/ Parkinson Disease, Secondary - genetics
/ Parkinson Disease, Secondary - metabolism
/ PC12细胞
/ Rats
/ Reactive Oxygen Species - metabolism
/ RNA, Small Interfering - genetics
/ Rotenone
/ Signal Transduction - drug effects
/ Vaccine
/ 信号通路
/ 神经保护作用
/ 类化合物
/ 细胞凋亡
/ 血红素加氧酶-1
/ 诱导
/ 鱼藤酮
