Journal Article

The plant WEE1 kinase is involved in checkpoint control activation in nematode induced galls

جامعة عين شمس - Ain Shams University [Cairo] (ASU), Vieira, Paulo, de Veylder, Lieven, Eekhout, Thomas, Embrapa Recursos Genéticos e Biotecnologia = Embrapa Genetic Resources & Biotechnology (CENARGEN) ; Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa), de Almeida Engler, Janice, Flanders Institute for Biotechnology, Laboratório de Interação Molecular Planta-Praga ; Embrapa Recursos Genéticos e Biotecnologia = Embrapa Genetic Resources & Biotechnology (CENARGEN) ; Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa)-Brazilian Agricultural Research Corporation = Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Rodiuc, Natalia, R. Coelho, Roberta, Cabral, Danila, Department of Plant Biotechnology and Genetics ; Universiteit Gent = Ghent University = Université de Gand (UGENT), Chevalier, Christian, Banora, Mohamed Youssef, Dijair Antonino, José, Engler, Gilbert, Institut Sophia Agrobiotech (ISA) ; Université Nice Sop

2020

Overview

Galls induced by plant-parasitic nematodes involve a hyperactivation of the plant mitotic and endocycle machinery for their profit. Dedifferentiation of host root cells includes drastic cellular and molecular readjustments. In such background, potential DNA damage in the genome of gall cells is eminent. We questioned if DNA damage checkpoints activation followed by DNA repair occurred, or was eventually circumvented, in nematode-induced galls. Galls display transcriptional activation of the DNA damage checkpoint kinase WEE1, correlated with its protein localization in the nuclei. The promoter of the stress marker gene SMR7 was evaluated under the WEE1-knockout background. Drugs inducing DNA damage and a marker for DNA repair, PARP1 were used to understand mechanisms that might cope with DNA damage in galls. Our functional study revealed that gall cells lacking WEE1 conceivably entered mitosis prematurely disturbing the cell cycle despite the loss of genome integrity. The disrupted nuclei phenotype in giant cells hinted to the accumulation of mitotic defects. As well, WEE1-knockout in Arabidopsis and downregulation in tomato repressed infection and reproduction of root-knot nematodes. Together with data on DNA damaging drugs, we suggest a conserved function for WEE1 controlling a G1/S cell cycle arrest in response to replication defect in galls.

• Publisher: Wiley,CCSD,Wiley Subscription Services, Inc
• Subject: animal reproduction / Arabidopsis / Arabidopsis thaliana / Cell cycle / cell cycle checkpoints / checkpoint control / Damage localization / Defects / Deoxyribonucleic acid / DNA / DNA damage / DNA repair / Drugs / enzymes / Gall / Galls / genetic markers / genome / Genomes / Giant cells / Kinases / Life Sciences / Localization / Markers / Mitosis / Nematodes / Nuclei / Nuclei (cytology) / Nucleus / phenotype / Phenotypes / plant root cells / Poly(ADP-ribose) polymerase / Repair / root-knot nematodes / root‐knot nematode / Solanum lycopersicum / Tomatoes / Transcription activation / transcriptional activation / Vegetal Biology / WEE1 kinase
• ISBN: 0004931729000