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Identification of novel PANDAR protein interaction partners involved in splicing regulation
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Identification of novel PANDAR protein interaction partners involved in splicing regulation
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Identification of novel PANDAR protein interaction partners involved in splicing regulation
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Journal Article
Identification of novel PANDAR protein interaction partners involved in splicing regulation
2018
Overview
Interactions of long non-coding RNAs (lncRNA) with proteins play important roles in the regulation of many cellular processes.
PANDAR
(
P
romotor of
CDKN1A
An
tisense
D
NA damage
A
ctivated
R
NA) is a lncRNA that is transcribed in a p53-dependent manner from the
CDKN1A
promoter and is involved in the regulation of proliferation and senescence. Overexpression of
PANDAR
has been observed in several tumor species and correlated with a poor prognosis for patient survival rate. Depending on the cellular state,
PANDAR
is known to interact with proteins such as the nuclear transcription factor Y subunit A (NF-YA) and the scaffold attachment factor A (SAF-A). However, a comprehensive analysis of the
PANDAR
interactome was missing so far. Therefore, we applied peptide nucleic acid (PNA)-based pull-downs combined with quantitative mass spectrometry to identify new protein binding partners. We confirmed potential candidates like U2AF65 and PTBP1, known to be involved in RNA processing. Furthermore, we observed that overexpression of
PANDAR
leads to a reduced level of the short pro-apoptotic
BCL-X
splice variant (
BCL-XS
) which is regulated by PTBP1. Simultaneous overexpression of PTBP1 was able to rescue this effect. Overall, our data suggest a role for
PANDAR
in the regulation of splicing events via its interaction partner PTBP1.
