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A class I cytosolic HSP20 of rice enhances heat and salt tolerance in different organisms
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A class I cytosolic HSP20 of rice enhances heat and salt tolerance in different organisms
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Journal Article
A class I cytosolic HSP20 of rice enhances heat and salt tolerance in different organisms
2020
Overview
Small heat shock proteins (sHSPs) have been thought to function as chaperones, protecting their targets from denaturation and aggregation when organisms are subjected to various biotic and abiotic stresses. We previously reported an sHSP from
Oryza sativa
(OsHSP20) that homodimerizes and forms granules within the cytoplasm but its function was unclear. We now show that OsHSP20 transcripts were significantly up-regulated by heat shock and high salinity but not by drought. A recombinant protein was purified and shown to inhibit the thermal aggregation of the mitochondrial malate dehydrogenase (MDH) enzyme
in vitro
, and this molecular chaperone activity suggested that OsHSP20 might be involved in stress resistance. Heterologous expression of OsHSP20 in
Escherichia coli
or
Pichia pastoris
cells enhanced heat and salt stress tolerance when compared with the control cultures. Transgenic rice plants constitutively overexpressing OsHSP20 and exposed to heat and salt treatments had longer roots and higher germination rates than those of control plants. A series of assays using its truncated mutants showed that its N-terminal arm plus the ACD domain was crucial for its homodimerization, molecular chaperone activity
in vitro
, and stress tolerance
in vivo
. The results supported the viewpoint that OsHSP20 could confer heat and salt tolerance by its molecular chaperone activity in different organisms and also provided a more thorough characterization of HSP20-mediated stress tolerance in
O. sativa
.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/111
/ 38/77
/ 38/90
/ 42/44
/ 82/80
/ 82/83
/ Drought
/ E coli
/ Escherichia coli - metabolism
/ Heat
/ HSP20 Heat-Shock Proteins - biosynthesis
/ HSP20 Heat-Shock Proteins - genetics
/ Humanities and Social Sciences
/ Microorganisms, Genetically-Modified - genetics
/ Microorganisms, Genetically-Modified - metabolism
/ Mutants
/ Plant Proteins - biosynthesis
/ Recombinant Proteins - biosynthesis
/ Recombinant Proteins - genetics
/ Salinity
/ Science
