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Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression
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Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression
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Journal Article
Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression
2016
Overview
The coordinated activity of DNA replication factors is a highly dynamic process that involves ubiquitin-dependent regulation. In this context, the ubiquitin-directed ATPase CDC-48/p97 recently emerged as a key regulator of chromatin-associated degradation in several of the DNA metabolic pathways that assure genome integrity. However, the spatiotemporal control of distinct CDC-48/p97 substrates in the chromatin environment remained unclear. Here, we report that progression of the DNA replication fork is coordinated by UBXN-3/FAF1. UBXN-3/FAF1 binds to the licensing factor CDT-1 and additional ubiquitylated proteins, thus promoting CDC-48/p97-dependent turnover and disassembly of DNA replication factor complexes. Consequently, inactivation of UBXN-3/FAF1 stabilizes CDT-1 and CDC-45/GINS on chromatin, causing severe defects in replication fork dynamics accompanied by pronounced replication stress and eventually resulting in genome instability. Our work identifies a critical substrate selection module of CDC-48/p97 required for chromatin-associated protein degradation in both
Caenorhabditis elegans
and humans, which is relevant to oncogenesis and aging.
Cdc48/p97 is a key component of the ubiquitin-proteasome system, acting as a ubiquitin-directed segregase to regulate multiple cellular functions. Here the authors identify UBXN-3/FAF1 as a crucial regulator of chromatin-associated protein degradation that recruits Cdc48/p97 to DNA replication forks.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
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/ 38/70
/ 64/11
/ 82/80
/ 82/83
/ Adaptor Proteins, Signal Transducing - genetics
/ Adaptor Proteins, Signal Transducing - metabolism
/ Adenosine Triphosphatases - genetics
/ Adenosine Triphosphatases - metabolism
/ Animals
/ Apoptosis Regulatory Proteins
/ Caenorhabditis elegans Proteins - genetics
/ Caenorhabditis elegans Proteins - metabolism
/ Cancer
/ Carrier Proteins - metabolism
/ Cell Cycle Proteins - genetics
/ Cell Cycle Proteins - metabolism
/ Chromosomal Proteins, Non-Histone - genetics
/ Chromosomal Proteins, Non-Histone - metabolism
/ DNA
/ Genomes
/ Humanities and Social Sciences
/ Humans
/ Licenses
/ Oncology
/ Proteins
/ Science
/ Yeast
