Journal Article

Crosstalk Between Adipokines and Paraoxonase 1: A New Potential Axis Linking Oxidative Stress and Inflammation

Cervellati, Carlo, Romani, Arianna, Milani, Daniela, Bonaccorsi, Gloria, Sanz, Juana M., Gemmati, Donato, Tisato, Veronica, Melloni, Elisabetta, Tavanti, Elisa, Passaro, Angelina

2019

Overview

Paraoxonase 1 (PON1) is a high-density lipoprotein (HDL)-associated protein that endows its carrier with (lipo-)lactonase-dependent antioxidative features. Low levels of PON1 activity have been observed in association with obesity, a major risk factor for cardiovascular disease (CVD). Considering the well-recognized atheroprotective role of PON1, exogenous/endogenous factors that might modulate its levels/activity are raising great interest. Since adipokines represent a molecular link between obesity and CVD, we here explored the possible impact of these substances on PON1 activity/expression. The levels of interleukin (IL)-6, IL-8, tumor necrosis factor alpha, monocyte chemoattractant protein-1, hepatocyte growth factor, resistin, leptin, and adiponectin were measured along with arylesterase, paraoxonase, and lactonase activities of PON1 in 107 postmenopausal women. Moreover, the direct effect of resistin on PON1 expression was evaluated in vitro. Multivariate analysis revealed that only resistin was significantly and inversely correlated with PON1-lactonase activities (r = −0.346, p < 0.001) regardless of confounding factors such as age or HDL-cholesterol. It is worth noting that no statistical link was found between adipokine and arylesterase or paraoxonase, the two promiscuous activities of PON1. Notably, resistin down-regulated PON1 expression occurred in hepatocellular carcinoma cultures. Our study suggests that resistin might be a negative modulator of PON1 expression and anti-oxidative activity.

• Publisher: MDPI AG,MDPI
• Subject: adipokines / Adiponectin / antioxidant activity / aryldialkylphosphatase / Arylesterase / Body fat / Cardiovascular diseases / chemokine CCL2 / Cholesterol / Cytokines / enzyme activity / Extinction / Hepatocellular carcinoma / Hepatocyte growth factor / hepatoma / High density lipoprotein / high density lipoprotein cholesterol / in vitro studies / Inflammation / Interleukin 8 / interleukin-6 / Leptin / Lipoproteins / Metabolism / Metabolites / Monocyte chemoattractant protein / Monocyte chemoattractant protein 1 / Monocytes / Multivariate analysis / Obesity / Oxidative stress / oxinflammation / Paraoxonase / Paraoxonase 1 / paraoxonase 1 (PON1) / Post-menopause / postmenopausal woman / postmenopause / resistin / Risk factors / tumor necrosis factor-alpha / Tumor necrosis factor-TNF / Tumor necrosis factor-α / women / Womens health