Journal Article

G-protein-coupled receptor GCR1 regulates DNA synthesis through activation of phosphatidylinositol-specific phospholipase C

Colucci, G, Alyeshmerni, N, Wiens, K, Chalmers, D, Apone, F, Chrispeels, M.J

2003

Overview

Different lines of evidence suggest that specific events during the cell cycle may be mediated by a heterotrimeric G-protein activated by a cognate G-protein coupled receptor. However, coupling between the only known Galpha-subunit of the heterotrimeric G-protein (GPA1) and the only putative G-protein coupled receptor (GCR1) of plants has never been shown. Using a variety of approaches, we show here that GCR1-enhanced thymidine incorporation into DNA depends on an increase in phosphatidylinositol-specific phospholipase C activity and an elevation of inositol 1,4,5-trisphosphate levels in the cells. Tobacco (Nicotiana tabacum) cells that overexpress either Arabidopsis GCR1 or GPA1 display this phenomenon. We suggest on the basis of these results that GCR1-controlled events during the cell cycle involve phosphatidylinositol-specific phospholipase C as an effector of GCR1 and inositol 1,4,5-trisphosphate as a second messenger, and that GCR1 and GPA1 are both involved in this particular signaling pathway.

• Publisher: American Society of Plant Biologists,American Society of Plant Physiologists,The American Society for Plant Biologists
• Subject: Animal cells / antagonists & inhibitors / Arabidopsis Proteins / Arabidopsis Proteins - metabolism / Arabidopsis thaliana / biochemical pathways / Biological and medical sciences / biosynthesis / Cell Biology and Signal Transduction / Cell cycle / Cell cycle proteins / Cell growth / Cell kinetics / Cell lines / Cell physiology / Cloning, Molecular / cultured cells / Deoxyribonucleic acid / DNA / DNA Replication / DNA Replication - genetics / DNA, Plant / DNA, Plant - biosynthesis / Enzyme Activation / enzyme inhibition / Enzyme Inhibitors / Enzyme Inhibitors - pharmacology / Estrenes / Estrenes - pharmacology / Fundamental and applied biological sciences. Psychology / G-protein-coupled receptor / G-proteins / GCR1 protein / Gene expression regulation / gene overexpression / genetics / GPA1 protein / Inositol 1,4,5-Trisphosphate / Inositol 1,4,5-Trisphosphate - metabolism / inositol phosphates / Inositols / metabolism / Models, Biological / Molecular and cellular biology / molecular sequence data / Nicotiana - metabolism / Nicotiana tabacum / nucleotide sequences / pharmacology / Phosphatidylinositol Diacylglycerol-Lyase / Phosphatidylinositol Diacylglycerol-Lyase - antagonists & inhibitors / Phosphatidylinositol Diacylglycerol-Lyase - metabolism / phosphatidylinositols / Phosphoinositide Phospholipase C / phospholipase C / Phosphorylation / Plant cells / Plant physiology and development / plant proteins / Protein Kinase C / Protein Kinase C - metabolism / protein kinases / Pyrrolidinones / Pyrrolidinones - pharmacology / Receptors / Receptors, G-Protein-Coupled / Receptors, G-Protein-Coupled - metabolism / Recombinant Proteins / Recombinant Proteins - metabolism / Signal Transduction / Thymidine / Thymidine - metabolism / Tobacco / Transcription Factors / Transcription Factors - metabolism / transgenes