Journal Article

O-serotype distribution of Escherichia coli bloodstream infection isolates in critically ill patients in The Netherlands

Morrow, B., Verboom, D.M., Varkila, M.R.J., Dudley, E.G., Cremer, O.L., Bonten, M.J.M., Davies, T., Ibarra de Palacios, P., Roberts, E., Poolman, J., Hermans, P.W.M.

2021

Overview

•The burden of invasive ExPEC infections is increasing.•A glycoconjugate vaccine, containing ten E. coli O-antigens, is in development.•Vaccine effectiveness will depend on serogroup distribution.•Common serogroups in ICU patients with invasive ExPEC were O25, O8, O2, O6, and O15.•Theoretical vaccine coverage was 54% in this population. Invasive infections by extra-intestinal pathogenic Escherichia coli (ExPEC) strains are increasing. We determined O-serogroups of E. coli isolates from ICU patients having bloodstream infections (BSI) and the potential coverage of a 10-valent O-polysaccharide conjugate vaccine currently in development for the prevention of invasive ExPEC disease. We studied E. coli BSI among patients admitted to a tertiary ICU in the Netherlands between April 2011 and November 2016. O-serogroups were determined in vitro by agglutination and whole genome sequencing. Among 714 ICU patients having BSI, 70 (10%) had an E. coli BSI. Among 68 (97%) isolates serogrouped, the most common serogroups were O25 (n = 11; 16%), O8 (n = 5; 7%), O2 (n = 4; 6%), O6 (n = 4; 6%), and O15 (n = 4; 6%). The theoretical coverage of a 10-valent ExPEC vaccine was 54% (n = 37). A multi-valent ExPEC O-polysaccharide conjugate vaccine in development could potentially aid in the prevention of E. coli BSI in Dutch ICU patients.

• Publisher: Elsevier Ltd,Elsevier Limited
• Subject: Abdomen / Abdominal surgery / Agglutination / Allergy and Immunology / Antibiotics / Antimicrobial agents / blood flow / Bloodstream infections / Conjugates / Drug resistance / E coli / Escherichia coli / ExPEC / extraintestinal pathogenic Escherichia coli / Gene sequencing / genome / Genomes / ICU / Intestine / Netherlands / Nosocomial infections / Pathogens / Patients / Polysaccharides / Prevention / Sepsis / serotypes / Urinary tract diseases / Urinary tract infections / Urogenital system / Vaccine / Vaccines / Whole genome sequencing