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Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
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Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
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Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
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Journal Article
Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica
2019
Overview
Recombinant protein production represents a multibillion-dollar market. Therefore, it constitutes an important research field both in academia and industry. The use of yeast as a cell factory presents several advantages such as ease of genetic manipulation, growth at high cell density, and the possibility of post-translational modifications. Yarrowia lipolytica is considered as one of the most attractive hosts due to its ability to metabolize raw substrate, to express genes at a high level, and to secrete protein in large amounts. In recent years, several reviews have been dedicated to genetic tools developed for this purpose. Though the construction of efficient cell factories for recombinant protein synthesis is important, the development of an efficient process for recombinant protein production in a bioreactor constitutes an equally vital aspect. Indeed, a sports car cannot drive fast on a gravel road. The aim of this review is to provide a comprehensive snapshot of process tools to consider for recombinant protein production in bioreactor using Y. lipolytica as a cell factory, in order to facilitate the decision-making for future strain and process engineering.
