Asset Details
Do you wish to reserve the book?
Toward the atomic structure of the nuclear pore complex: when top down meets bottom up
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Toward the atomic structure of the nuclear pore complex: when top down meets bottom up
Do you wish to request the book?
Toward the atomic structure of the nuclear pore complex: when top down meets bottom up
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Toward the atomic structure of the nuclear pore complex: when top down meets bottom up
2016
Overview
This Perspective discusses how two complementary approaches, bottom-up
in vitro
and top-down
in situ
structural biology, have now converged to generate the first predictive structural models of the nuclear pore scaffold.
Elucidating the structure of the nuclear pore complex (NPC) is a prerequisite for understanding the molecular mechanism of nucleocytoplasmic transport. However, owing to its sheer size and flexibility, the NPC is unapproachable by classical structure determination techniques and requires a joint effort of complementary methods. Whereas bottom-up approaches rely on biochemical interaction studies and crystal-structure determination of NPC components, top-down approaches attempt to determine the structure of the intact NPC
in situ
. Recently, both approaches have converged, thereby bridging the resolution gap from the higher-order scaffold structure to near-atomic resolution and opening the door for structure-guided experimental interrogations of NPC function.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Analysis
/ Crystals
/ Humans
/ Molecular Chaperones - chemistry
/ Molecular Chaperones - genetics
/ Molecular Chaperones - metabolism
/ Nuclear Pore - ultrastructure
/ Nuclear Pore Complex Proteins - chemistry
/ Nuclear Pore Complex Proteins - genetics
/ Nuclear Pore Complex Proteins - metabolism
/ Protein Isoforms - chemistry
/ Protein Isoforms - metabolism
/ Protein Structure, Secondary
/ Saccharomyces cerevisiae - genetics
/ Saccharomyces cerevisiae - metabolism
/ Saccharomyces cerevisiae - ultrastructure
/ Saccharomyces cerevisiae Proteins - chemistry
/ Saccharomyces cerevisiae Proteins - genetics
