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Fibroblast Activation Protein-Targeted CAR-T Cells Induce Apoptosis in Murine Cardiac Myofibroblasts
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Fibroblast Activation Protein-Targeted CAR-T Cells Induce Apoptosis in Murine Cardiac Myofibroblasts
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Journal Article
Fibroblast Activation Protein-Targeted CAR-T Cells Induce Apoptosis in Murine Cardiac Myofibroblasts
2025
Overview
Myocardial fibrosis is a common pathological feature in many cardiovascular diseases, yet effective targeted therapies remain elusive. Given the emerging potential of chimeric antigen receptor T (CAR-T) cell therapy in nononcological diseases and fibroblast activation protein (FAP) as a promising target, we engineered a second-generation FAP-targeted CAR construct incorporating the 4-1BB costimulatory domain to enhance therapeutic safety. Using two delivery approaches—lentiviral vectors and lipid nanoparticles (LNPs)—we generated FAP-CAR–engineered Jurkat cells as a preliminary screening model and evaluated their CAR expression, target recognition, and in vitro cytotoxic activity. These engineered cells selectively recognized and induced apoptosis in FAP-expressing cardiac myofibroblasts without triggering excessive IL-6 secretion, supporting their potential for fibrosis-selective cytotoxicity. Our findings provide key preliminary in vitro evidence supporting the design and target-specific functionality of FAP-targeted CAR constructs incorporating the 4-1BB domain, warranting further investigation in primary T cell models for cardiac fibrosis therapy.
Publisher
Wiley,John Wiley & Sons, Inc
Subject
/ Antigens
/ Fibrosis
/ Humans
/ Immunotherapy, Adoptive - methods
/ Membrane Proteins - genetics
/ Membrane Proteins - immunology
/ Membrane Proteins - metabolism
/ Mice
/ Receptors, Chimeric Antigen - genetics
/ Receptors, Chimeric Antigen - immunology
/ Receptors, Chimeric Antigen - metabolism
/ Serine Endopeptidases - genetics
/ Serine Endopeptidases - immunology
/ Serine Endopeptidases - metabolism
/ T cells
/ T-Lymphocytes - transplantation
/ Tumor Necrosis Factor Receptor Superfamily, Member 9 - genetics
/ Tumor Necrosis Factor Receptor Superfamily, Member 9 - metabolism
