Journal Article

Absence of Proteinase-Activated Receptor-1 Signaling in Mice Confers Protection from fMLP-Induced Goblet Cell Metaplasia

Lucattelli, Monica, Scotton, Chris J, De Cunto, Giovanna, Laurent, Geoffrey J, Chambers, Rachel C, Bartalesi, Barbara, Lungarella, Giuseppe, Lunghi, Benedetta, Atzori, Luigi

2009

Overview

Abstract The morphological features of chronic obstructive pulmonary disease in man include emphysema and chronic bronchitis associated with mucus hypersecretion. These alterations can be induced in mice by a single intratracheal instillation of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), a chemoattractant and degranulating agent for neutrophils. The mechanisms underlying excessive mucus production and, in particular, goblet cell hyperplasia/metaplasia in chronic obstructive pulmonary disease remain poorly understood. The proteinase-activated receptors (PARs) are widely recognized for their modulatory properties during inflammation. In this study, we examined whether PAR-1 contributes to inflammation and lung damage induced by fMLP by comparing the response of PAR-1–deficient (PAR-1−/−) mice with that of wild-type (WT) mice. Mice were killed at various time points after fMLP instillation (200 μg/50 μl). WT mice developed emphysema and goblet cell metaplasia. The onset of pulmonary lesions was preceded by an increase in thrombin immunoreactivity in bronchial airways and alveolar tissue. This was followed by a decrease in PAR-1 immunoreactivity, and by an increase in IL-13 immunostaining on the luminal surface of airway epithelial cells. In PAR-1−/− mice, fMLP administration induced similar responses in terms of inflammation and emphysema, but these mice were protected from the development of goblet cell metaplasia. The involvement of PAR-1 in airway epithelial cell transdifferentiation was confirmed by demonstrating that intratracheal instillation of the selective PAR-1 agonist (TFLLR) induced goblet cell metaplasia in the airways of WT mice only. These data suggest that emphysema and goblet cell metaplasia occur independently, and that PAR-1 signaling through IL-13 stimulation may play an important role in inducing goblet cell metaplasia.

• Publisher: Am Thoracic Soc,Oxford University Press,American Thoracic Society
• Subject: Alveoli / Animals / Bronchitis / Cell Differentiation - drug effects / Chronic obstructive pulmonary disease / Emphysema / Emphysema - chemically induced / Emphysema - metabolism / Emphysema - pathology / Epithelial cells / ErbB Receptors - metabolism / Formyl peptides / Goblet Cells - drug effects / Goblet Cells - metabolism / Goblet Cells - pathology / Humans / Hyperplasia / Immunoreactivity / Inflammation / Interleukin 13 / Interleukin-13 - metabolism / Leukocytes (neutrophilic) / Lung - drug effects / Lung - metabolism / Lung - pathology / Lung diseases / Male / Metaplasia / Mice / Mice, Inbred C57BL / Mice, Knockout / Mucus / N-Formylmethionine Leucyl-Phenylalanine - toxicity / Oligopeptides - pharmacology / Phenylalanine / Proteinase-activated receptor 1 / Pulmonary Disease, Chronic Obstructive - etiology / Pulmonary lesions / Receptor mechanisms / Receptor, PAR-1 - agonists / Receptor, PAR-1 - deficiency / Receptor, PAR-1 - genetics / Respiratory tract / Signal Transduction / Thrombin / Trachea