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Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms

by Cavaliere Rosalia , Nomura Nobuhiko , Toyofuku Masanori , Turnbull Lynne , Hynen Amelia L. , Pessi Gabriella , 豊福雅典 , Monahan Leigh G. , Osvath Sarah R. , Yap Xinhui , Shimoni Raz , Omasits Ulrich , Ahrens Christian H. , Whitchurch Cynthia B. , Petty Nicola K. , Kurosawa Masaharu , Eberl Leo , Gloag Erin S. , Ito Satoshi , Ca'rcamo-Oyarce Gerardo , Charles Ian G.

in 14 / 14/34 / 14/63 / 38 / 38/35 / 38/91 / 631/326/41/88 / 631/326/46 / Bacteria / Bacteriolysis - drug effects / Biofilms / Biofilms - drug effects / Bioinformatics / Biology / Biosynthesis / Cell division / Cell Membrane - drug effects / Cell Membrane - metabolism / Deoxyribonucleic acid / DNA / DNA, Bacterial - metabolism / Endopeptidases - pharmacology / Extracellular Space - metabolism / Gram-positive bacteria / Humanities and Social Sciences / multidisciplinary / Organelle Biogenesis / Proteins / Pseudomonas aeruginosa - drug effects / Pseudomonas aeruginosa - physiology / Pyocins - pharmacology / Quinolones - pharmacology / Science / Science (multidisciplinary) / Stress, Physiological - drug effects / Virulence

2016

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Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms

2016

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Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms

2016

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Journal Article

Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms

Cavaliere Rosalia,

Nomura Nobuhiko,

Toyofuku Masanori,

Turnbull Lynne,

Hynen Amelia L.,

Pessi Gabriella,

豊福雅典,

Monahan Leigh G.,

Osvath Sarah R.,

Yap Xinhui,

Shimoni Raz,

Omasits Ulrich,

Ahrens Christian H.,

Whitchurch Cynthia B.,

Petty Nicola K.,

Kurosawa Masaharu,

Eberl Leo,

Gloag Erin S.,

Ito Satoshi,

Ca'rcamo-Oyarce Gerardo,

Charles Ian G.

2016

Overview

Many bacteria produce extracellular and surface-associated components such as membrane vesicles (MVs), extracellular DNA and moonlighting cytosolic proteins for which the biogenesis and export pathways are not fully understood. Here we show that the explosive cell lysis of a sub-population of cells accounts for the liberation of cytosolic content in Pseudomonas aeruginosa biofilms. Super-resolution microscopy reveals that explosive cell lysis also produces shattered membrane fragments that rapidly form MVs. A prophage endolysin encoded within the R- and F-pyocin gene cluster is essential for explosive cell lysis. Endolysin-deficient mutants are defective in MV production and biofilm development, consistent with a crucial role in the biogenesis of MVs and liberation of extracellular DNA and other biofilm matrix components. Our findings reveal that explosive cell lysis, mediated through the activity of a cryptic prophage endolysin, acts as a mechanism for the production of bacterial MVs.

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Publisher

Nature Publishing Group,Nature Publishing Group UK,Nature Portfolio

Subject

/ 14/34

/ 14/63

/ 38

/ 38/35

/ 38/91

/ 631/326/41/88

/ 631/326/46