Journal Article

Genetic Architecture of Dietary Fiber and Oligosaccharide Content in a Middle American Panel of Edible Dry Bean

Thompson, Henry J., Moghaddam, Samira Mafi, Brick, Mark A., Echeverria, Dimas, Lee, Rian, Mamidi, Sujan, McClean, Phillip E., Brick, Leslie A.

2018

Overview

Core Ideas Colored bean seed showed the highest amount of IDF Over 20 years of breeding, higher levels of IDF and RFOs were achieved unintentionally Strong candidate genes were identified for dietary fiber traits especially RFO component Common bean (Phaseolus vulgaris L.) is the most consumed edible grain legume worldwide and contains a wide range of nutrients for human health including dietary fiber. Diets high in beans are associated with lower rates of chronic diseases such as obesity and type 2 diabetes, and the content of dietary fibers varies among different market classes of dry bean. In this study, we evaluated the dietary fiber content in a Middle American diversity panel (MDP) of common bean and evaluated the genetic architecture of the various dietary fiber components. The dietary fiber components included insoluble and soluble dietary fibers as well as the antinutritional raffinose family of oligosaccharides (RFOs; raffinose, stachyose, and verbascose). All variables measured differed among market classes and entries. Colored bean seeds had higher levels of insoluble dietary fibers with the black market class showing also the highest raffinose and stachyose content. Cultivars and lines released since 1997 had higher insoluble dietary fibers and RFO content in race Durango. Higher levels of RFOs were also observed in cultivars with type II growth habit that was a recent breeding target in Durango race germplasm. Candidate genes for dietary fiber traits, especially homologs to two main genes in the RFO biosynthesis pathway, were identified. The knowledge of diversity of dietary fibers in the MDP accompanied with the identification of candidate genes could effectively improve dietary fiber components in common bean.

• Publisher: Crop Science Society of America,John Wiley & Sons, Inc,Wiley
• Subject: Beans / biosynthesis / chronic diseases / Chronic illnesses / color / Crop diseases / Crops / Cultivars / Diabetes / Diabetes mellitus (non-insulin dependent) / Diet / Dietary fiber / dry beans / fiber content / Fibers / Genes / Genomes / Germplasm / growth habit / human health / Legumes / markets / noninsulin-dependent diabetes mellitus / nutrients / Obesity / Oligosaccharides / Phaseolus vulgaris / Plant breeding / Prebiotics / Raffinose / Seeds / Stachyose