Asset Details
Do you wish to reserve the book?
Botulinum Neurotoxin Is Shielded by NTNHA in an Interlocked Complex
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Botulinum Neurotoxin Is Shielded by NTNHA in an Interlocked Complex
Do you wish to request the book?
Botulinum Neurotoxin Is Shielded by NTNHA in an Interlocked Complex
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Botulinum Neurotoxin Is Shielded by NTNHA in an Interlocked Complex
2012
Overview
Botulinum neurotoxins (BoNTs) are highly poisonous substances that are also effective medicines. Accidental BoNT poisoning often occurs through ingestion of Clostrídium botulinum-contaminated food. Here, we present the crystal structure of a BoNT in complex with a clostridial nontoxic nonhemagglutinin (NTNHA) protein at 2.7 angstroms. Biochemical and functional studies show that NTNHA provides large and multivalent binding interfaces to protect BoNT from gastrointestinal degradation. Moreover, the structure highlights key residues in BoNT that regulate complex assembly in a pH-dependent manner. Collectively, our findings define the molecular mechanisms by which NTNHA shields BoNT in the hostile gastrointestinal environment and releases it upon entry into the circulation. These results will assist in the design of small molecules for inhibiting oral BoNT intoxication and of delivery vehicles for oral administration of biologies.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - metabolism
/ Biological and medical sciences
/ Botulinum Toxins, Type A - chemistry
/ Botulinum Toxins, Type A - metabolism
/ DESIGN
/ DRUGS
/ FOOD
/ Fundamental and applied biological sciences. Psychology
/ Multiprotein Complexes - chemistry
/ Multiprotein Complexes - metabolism
/ Neurons
/ Poisons
/ Protein Interaction Domains and Motifs
/ Protein Structure, Secondary
/ PROTEINS
/ RESIDUES
/ SHIELDS
/ Structure in molecular biology
/ Toxins
