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Determining the polysaccharide composition of plant cell walls

by Bacic, Antony , Pettolino, Filomena A , Fincher, Geoffrey B , Walsh, Cherie

in 101/58 / 631/1647/2196 / 631/449/448/1365 / 631/45/72/1205 / Acetic acid / Agriculture / Analytic Sample Preparation Methods - methods / Analytical Chemistry / Biofuels / Biological Techniques / Biomedical materials / Cell Wall - chemistry / Cell walls / Cellulose / Composite materials / Composition / Computational Biology/Bioinformatics / Dietary fiber / Fabrics / Fine chemicals / Gas chromatography / Gas Chromatography-Mass Spectrometry - methods / Life Sciences / Lignin / Linkage analysis / Mass spectrometry / Mass spectroscopy / Methylation / Microarrays / Monosaccharides / Organic Chemistry / Physiological aspects / Plant biomass / Plants - chemistry / Polysaccharides / Polysaccharides - analysis / Protocol / Saccharides / Sugar

2012

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Determining the polysaccharide composition of plant cell walls

by Bacic, Antony , Pettolino, Filomena A , Fincher, Geoffrey B , Walsh, Cherie

2012

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Determining the polysaccharide composition of plant cell walls

by Bacic, Antony , Pettolino, Filomena A , Fincher, Geoffrey B , Walsh, Cherie

2012

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Journal Article

Determining the polysaccharide composition of plant cell walls

Bacic, Antony,

Pettolino, Filomena A,

Fincher, Geoffrey B,

Walsh, Cherie

2012

Overview

The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocomposite industries. We present analytical techniques not only to define the fine chemical structures of individual cell wall polysaccharides but also to estimate the overall polysaccharide composition of cell wall preparations. The procedure covers the preparation of cell walls, together with gas chromatography–mass spectrometry (GC-MS)-based methods, for both the analysis of monosaccharides as their volatile alditol acetate derivatives and for methylation analysis to determine linkage positions between monosaccharide residues as their volatile partially methylated alditol acetate derivatives. Analysis time will vary depending on both the method used and the tissue type, and ranges from 2 d for a simple neutral sugar composition to 2 weeks for a carboxyl reduction/methylation linkage analysis.

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Publisher

Nature Publishing Group UK,Nature Publishing Group

Subject

/ Analytic Sample Preparation Methods - methods