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Identification, Biochemical Characterization, and Subcellular Localization of Allantoate Amidohydrolases from Arabidopsis and Soybean
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Identification, Biochemical Characterization, and Subcellular Localization of Allantoate Amidohydrolases from Arabidopsis and Soybean
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Identification, Biochemical Characterization, and Subcellular Localization of Allantoate Amidohydrolases from Arabidopsis and Soybean
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Journal Article
Identification, Biochemical Characterization, and Subcellular Localization of Allantoate Amidohydrolases from Arabidopsis and Soybean
2008
Overview
Allantoate amidohydrolases (AAHs) hydrolize the ureide allantoate to ureidoglycolate, CO₂, and two molecules of ammonium. Allantoate degradation is required to recycle purine-ring nitrogen in all plants. Tropical legumes additionally transport fixed nitrogen via allantoin and allantoate into the shoot, where it serves as a general nitrogen source. AAHs from Arabidopsis (Arabidopsis thaliana; AtAAH) and from soybean (Glycine max; GmAAH) were cloned, expressed in planta as StrepII-tagged variants, and highly purified from leaf extracts. Both proteins form homodimers and release 2 mol ammonium/mol allantoate. Therefore, they can truly be classified as AAHs. The kinetic constants determined and the half-maximal activation by 2 to 3 μM manganese are consistent with allantoate being the in vivo substrate of manganese-loaded AAHs. The enzymes were strongly inhibited by micromolar concentrations of fluoride as well as by borate, and by millimolar concentrations of L-asparagine and L-aspartate but not D-asparagine. L-Asparagine likely functions as competitive inhibitor. An Ataah T-DNA mutant, unable to grow on allantoin as sole nitrogen source, is rescued by the expression of StrepII-tagged variants of AtAAH and GmAAH, demonstrating that both proteins are functional in vivo. Similarly, an allantoinase (aln) mutant is rescued by a tagged AtAln variant. Fluorescent fusion proteins of allantoinase and both AAHs localize to the endoplasmic reticulum after transient expression and in transgenic plants. These findings demonstrate that after the generation of allantoin in the peroxisome, plant purine degradation continues in the endoplasmic reticulum.
Publisher
American Society of Plant Biologists,American Society of Plant Physiologists
Subject
/ Amidohydrolases - metabolism
/ Biochemical Processes and Macromolecular Structures
/ Biological and medical sciences
/ Borates
/ Enzymes
/ Escherichia coli - metabolism
/ Fundamental and applied biological sciences. Psychology
/ Gels
/ Gene Expression Regulation, Plant
/ Gene Expression Regulation, Plant - genetics
/ Gene Expression Regulation, Plant - physiology
/ Genetic Complementation Test
/ genetics
/ Kinetics
/ Metals
/ mutants
/ Mutation
/ Nitrogen
/ Plant physiology and development
/ Plants
/ proteins
/ Quaternary ammonium compounds
/ shoots
/ Soybeans
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