Journal Article

Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome

Graves, Lee M., Webster, Megan J., Livraghi-Butrico, Alessandra, Dang, Hong, Rogers, Keith, Ghosh, Arunava, Randell, Scott H., Herring, Laura E., Davis, Eric S., Mascenik, Teresa, Rowell, Temperance R., Sassano, M. Flori, Alexis, Neil E., Van Buren, Scott K., Coakley, Raymond C., Tarran, Robert, Herman, Melissa A.

2018

Overview

E-cigarettes vaporize propylene glycol/vegetable glycerin (PG/VG), nicotine, and flavorings. However, the long-term health effects of exposing lungs to vaped e-liquids are unknown. To determine the effects of chronic vaping on pulmonary epithelia. We performed research bronchoscopies on healthy nonsmokers, cigarette smokers, and e-cigarette users (vapers) and obtained bronchial brush biopsies and lavage samples from these subjects for proteomic investigation. We further employed in vitro and murine exposure models to support our human findings. Visual inspection by bronchoscopy revealed that vaper airways appeared friable and erythematous. Epithelial cells from biopsy samples revealed approximately 300 proteins that were differentially expressed in smoker and vaper airways, with only 78 proteins being commonly altered in both groups and 113 uniquely altered in vapers. For example, CYP1B1 (cytochrome P450 family 1 subfamily B member 1), MUC5AC (mucin 5 AC), and MUC4 levels were increased in vapers. Aerosolized PG/VG alone significantly increased MUC5AC protein in human airway epithelial cultures and in murine nasal epithelia in vivo. We also found that e-liquids rapidly entered cells and that PG/VG reduced membrane fluidity and impaired protein diffusion. We conclude that chronic vaping exerts marked biological effects on the lung and that these effects may in part be mediated by the PG/VG base. These changes are likely not harmless and may have clinical implications for the development of chronic lung disease. Further studies will be required to determine the full extent of vaping on the lung.

• Publisher: Oxford University Press,American Thoracic Society
• Subject: Adult / Aquaporins / Biomarkers / Biopsy / Bronchi - drug effects / Cancer / Chronic obstructive pulmonary disease / Cytochrome / Deoxyribonucleic acid / DNA / Electronic cigarettes / Electronic Nicotine Delivery Systems / Enzymes / Epithelial Cells - drug effects / Female / Gene expression / Health care / Humans / Inflammation / Lung - drug effects / Lungs / Male / Middle Aged / Nicotine / Nicotine - adverse effects / Original / Oxidative stress / Proteins / Proteome - drug effects / Smokers / Smoking / Vaping