Asset Details

MbrlCatalogueTitleDetail

Do you wish to reserve the book?

Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis

by Nielsen, Jens , Darbani, Behrooz , Boles, Eckhard , Nour-Eldin, Hussam H , Borodina, Irina , Møller-Hansen, Iben , Belew, Zeinu M , Linder, Tomas , Tiukova, Ievgeniia A

in Ascomycota / Base Sequence / bioethanol / Biological Transport / Brettanomyces - classification / Brettanomyces - genetics / Brettanomyces - metabolism / Brettanomyces bruxellensis / Carbohydrate Metabolism / Cloning, Molecular / Ethanol - metabolism / Fermentation / Fungi / Gene Expression / Genetics / Genetik / Glucose - metabolism / glucose transport / Glucose Transport Proteins, Facilitative - chemistry / Glucose Transport Proteins, Facilitative - genetics / Glucose Transport Proteins, Facilitative - metabolism / high-affinity / metabolism / Microbiology / Mikrobiologi / Multigene Family / Observations / Oocytes - metabolism / Phylogeny / Physiological aspects / Research Letter / Sequence Analysis, Protein / Xenopus laevis / yeast

2019

Yes Please

Hey, we have placed the reservation for you!

By the way, why not check out events that you can attend while you pick your title.

Oops! Something went wrong.

Looks like we were not able to place the reservation. Kindly try again later.

Are you sure you want to remove the book from the shelf?

Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis

by Nielsen, Jens , Darbani, Behrooz , Boles, Eckhard , Nour-Eldin, Hussam H , Borodina, Irina , Møller-Hansen, Iben , Belew, Zeinu M , Linder, Tomas , Tiukova, Ievgeniia A

2019

Confirm

Do you wish to request the book?

Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis

by Nielsen, Jens , Darbani, Behrooz , Boles, Eckhard , Nour-Eldin, Hussam H , Borodina, Irina , Møller-Hansen, Iben , Belew, Zeinu M , Linder, Tomas , Tiukova, Ievgeniia A

2019

Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy

How would you like to get it?

Submit

We have requested the book for you!

Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.

Oops! Something went wrong.

Looks like we were not able to place your request. Kindly try again later.

Journal Article

Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis

Nielsen, Jens,

Darbani, Behrooz,

Boles, Eckhard,

Nour-Eldin, Hussam H,

Borodina, Irina,

Møller-Hansen, Iben,

Belew, Zeinu M,

Linder, Tomas,

Tiukova, Ievgeniia A

2019

Overview

The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.

Share this book

Add to My Shelf

Publisher

Oxford University Press

Subject

Ascomycota

/ Base Sequence

/ bioethanol

/ Biological Transport

/ Brettanomyces - classification

/ Brettanomyces - genetics

/ Brettanomyces - metabolism