Journal Article

Influence of Estradiol-17beta on Progesterone and Estrogen Receptor mRNA Expression in Porcine Follicular Granulosa Cells during Short-Term, In Vitro Real-Time Cell Proliferation

Dyszkiewicz Konwińska, Marta, Brüssow, Klaus P., Jopek, Karol, Budna, Joanna, Ciesiółka, Sylwia, Borys, Sylwia, Chachuła, Adrian, Ziolkowska, Agnieszka, Nowicki, Michał, Bryja, Artur, Bruska, Małgorzata, Antosik, Paweł, Kranc, Wiesława, Kempisty, Bartosz, Bukowska, Dorota, Zabel, Maciej

2016

Overview

Progesterone (P4) and estradiol (E2) play a significant role in mammalian reproduction. Our study demonstrated that separated porcine cumulus cells (CCs) and/or granulosa cells (GCs) might proliferate in vitro during short-term, real-time primary culture. The GCs were analyzed according to gene expression of the progesterone receptor (nuclear form) (pgr), progesterone receptor membrane component 1 (pgrmc1), and estrogen-related receptor beta 3 (esrrb3) in relation to two housekeeping genes: actb and pbgd. GCs were cultivated in medium with the E2. Both pgr/actb and pgr/pbgd revealed higher expression between 24 and 168 h of IVC of prolonged E2 treatment and at 48 h of IVC after acute E2 administration. The pgrmc1/actb and pgrmc1/pbgd displayed increased expression after prolonged E2 treatment between 24 and 120 h of IVC. The highest level of esrrb3/actb at 120 and 144 h, as well as esrrb3/pbgd at 120 h, in untreated controls as compared to the hormone-stimulated group, was observed. We suggest that E2 significantly influences the upregulation of pgr, pgrmc1, and esrrb3 expression in porcine GCs during real-time cell proliferation. Since esrrb3 expression is stimulated by E2 in both an acute and prolonged manner, estradiol may be recognized as a potential estrogen receptor agonist in GCs.

• Publisher: Hindawi Publishing Corporation,John Wiley & Sons, Inc
• Subject: Animals / Biomedical research / Cell cycle / Cell growth / Cell proliferation / Cell Proliferation - drug effects / Cell Proliferation - physiology / Cells, Cultured / Colleges & universities / Cytoplasm / Embryology / Estradiol / Estradiol - administration & dosage / Estrogens / Experiments / Female / Gene expression / Gene Expression Regulation - drug effects / Gene Expression Regulation - physiology / Genetic aspects / Granulosa Cells - cytology / Granulosa Cells - drug effects / Granulosa Cells - metabolism / Health aspects / Histology / Kinases / Messenger RNA / Progesterone / Proteins / Receptors, Estrogen - metabolism / Receptors, Progesterone - metabolism / RNA, Messenger - metabolism / Signal transduction / Swine / Time Factors