Asset Details

MbrlCatalogueTitleDetail

Do you wish to reserve the book?

Improved dominant selection markers and co-culturing conditions for efficient Agrobacterium tumefaciens-mediated transformation of Ustilago scitaminea

by Jiang, Zide , Ji, Lianghui , Liao, Jinling , Yan, Meixin , Ding, Zhaojian , Du, Minge , Xi, Pinggen , Liu, Yanbin , Sun, Longhua

in Agricultural biotechnology / Agrobacterium / Agrobacterium tumefaciens / Agrobacterium tumefaciens - genetics / Agrobacterium tumefaciens - growth & development / Applied Microbiology / Bacteria / Biochemistry / Biomedical and Life Sciences / Biotechnology / Culture / gene expression / Gene Transfer Techniques / Genes / Genetic markers / genetic transformation / Genetics / growth & development / Life Sciences / Markers / Microbiology / Moisture content / Mutagenesis / Original Research Paper / Pathogens / Plant diseases / plant pathogens / Selection, Genetic / smut diseases / Transformation, Genetic / Transformations / Ustilago / Ustilago - genetics / Ustilago - growth & development / Ustilago scitaminea / Water content

2014

Yes Please

Hey, we have placed the reservation for you!

By the way, why not check out events that you can attend while you pick your title.

Oops! Something went wrong.

Looks like we were not able to place the reservation. Kindly try again later.

Are you sure you want to remove the book from the shelf?

Improved dominant selection markers and co-culturing conditions for efficient Agrobacterium tumefaciens-mediated transformation of Ustilago scitaminea

by Jiang, Zide , Ji, Lianghui , Liao, Jinling , Yan, Meixin , Ding, Zhaojian , Du, Minge , Xi, Pinggen , Liu, Yanbin , Sun, Longhua

2014

Confirm

Do you wish to request the book?

Improved dominant selection markers and co-culturing conditions for efficient Agrobacterium tumefaciens-mediated transformation of Ustilago scitaminea

by Jiang, Zide , Ji, Lianghui , Liao, Jinling , Yan, Meixin , Ding, Zhaojian , Du, Minge , Xi, Pinggen , Liu, Yanbin , Sun, Longhua

2014

Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy

How would you like to get it?

Submit

We have requested the book for you!

Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.

Oops! Something went wrong.

Looks like we were not able to place your request. Kindly try again later.

Journal Article

Improved dominant selection markers and co-culturing conditions for efficient Agrobacterium tumefaciens-mediated transformation of Ustilago scitaminea

Jiang, Zide,

Ji, Lianghui,

Liao, Jinling,

Yan, Meixin,

Ding, Zhaojian,

Du, Minge,

Xi, Pinggen,

Liu, Yanbin,

Sun, Longhua

2014

Overview

Ustilago scitaminea is the causal agent of sugar-cane smut disease. There is, however, no genetic transformation method for it. Here we report the development of an efficient mutagenesis method based on Agrobacterium tumefaciens-mediated transformation. To improve transformation efficiency, a range of conditions, including the codon-usage preference of the selection marker gene, promoters and the culture conditions for transformation were optimized. A strong promoter to drive marker gene expression, optimized codon usage of selection marker gene, controlled water content and pH of co-culture medium were critical factors affecting transformation efficiency. Our findings provide a useful tool for genetic analysis of this important plant pathogen.

Share this book

Add to My Shelf

Publisher

Springer-Verlag,Springer Netherlands,Springer Nature B.V

Subject

Agricultural biotechnology

/ Agrobacterium

/ Agrobacterium tumefaciens

/ Agrobacterium tumefaciens - genetics

/ Agrobacterium tumefaciens - growth & development

/ Applied Microbiology

/ Bacteria