Asset Details
Do you wish to reserve the book?
Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function
Do you wish to request the book?
Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function
2024
Overview
Otolith organs in the inner ear and neuromasts in the fish lateral-line harbor two populations of hair cells oriented to detect stimuli in opposing directions. The underlying mechanism is highly conserved: the transcription factor EMX2 is regionally expressed in just one hair cell population and acts through the receptor GPR156 to reverse cell orientation relative to the other population. In mouse and zebrafish, loss of Emx2 results in sensory organs that harbor only one hair cell orientation and are not innervated properly. In zebrafish, Emx2 also confers hair cells with reduced mechanosensory properties. Here, we leverage mouse and zebrafish models lacking GPR156 to determine how detecting stimuli of opposing directions serves vestibular function, and whether GPR156 has other roles besides orienting hair cells. We find that otolith organs in Gpr156 mouse mutants have normal zonal organization and normal type I-II hair cell distribution and mechano-electrical transduction properties. In contrast, gpr156 zebrafish mutants lack the smaller mechanically evoked signals that characterize Emx2-positive hair cells. Loss of GPR156 does not affect orientation-selectivity of afferents in mouse utricle or zebrafish neuromasts. Consistent with normal otolith organ anatomy and afferent selectivity, Gpr156 mutant mice do not show overt vestibular dysfunction. Instead, performance on two tests that engage otolith organs is significantly altered – swimming and off-vertical-axis rotation. We conclude that GPR156 relays hair cell orientation and transduction information downstream of EMX2, but not selectivity for direction-specific afferents. These results clarify how molecular mechanisms that confer bi-directionality to sensory organs contribute to function, from single hair cell physiology to animal behavior.
Publisher
eLife Science Publications, Ltd,eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
Subject
/ Analysis
/ Animals
/ Hair Cells, Auditory - physiology
/ Homeodomain Proteins - genetics
/ Homeodomain Proteins - metabolism
/ Lateral Line System - embryology
/ Lateral Line System - physiology
/ mechano-electrical transduction
/ Mechanotransduction, Cellular
/ Mice
/ Mutants
/ Optical instruments industry
/ Otolithic Membrane - physiology
/ Proteins
/ Rodents
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Utricle
