Journal Article

Synergy between Piezo1 and Piezo2 channels confers high-strain mechanosensitivity to articular cartilage

Jeffrey Coles, Farshid Guilak, Amy L. McNulty, Suk Hee Lee, Stefan Zauscher, Wolfgang B. Liedtke, Nicole A. Zelenski, Jörg Grandl, Frederick Sachs, Jason Wu, Kellie N. Beicker, Whasil Lee, Holly A. Leddy, Yong Chen

2014

Overview

Significance Cartilage, a mechanically sensitive tissue that covers joints, is essential for vertebrate locomotion by sustaining skeletal mobility. Transduction of mechanical stimuli by cartilage cells, chondrocytes, leads to biochemical–metabolic responses. Such mechanotransduction can be beneficial for tissue maintenance when evoked by low-level mechanical stimuli, or can have health-adverse effects via cartilage-damaging high-strain mechanical stress. Thus, high-strain mechanotransduction by cartilage mechanotrauma is relevant for the pathogenesis of osteoarthritis. Molecular mechanisms of high-strain mechanotransduction of chondrocytes have been elusive. Here we identify Piezo1 and Piezo2 mechanosensitive ion channels in chondrocytes as transduction channels for high-strain mechanical stress. We verify their functional link to the cytoskeleton as important for their concerted function and offer a remedial strategy by application of a Piezo1/2 blocking peptide, GsMTx4, from tarantula venom. Diarthrodial joints are essential for load bearing and locomotion. Physiologically, articular cartilage sustains millions of cycles of mechanical loading. Chondrocytes, the cells in cartilage, regulate their metabolic activities in response to mechanical loading. Pathological mechanical stress can lead to maladaptive cellular responses and subsequent cartilage degeneration. We sought to deconstruct chondrocyte mechanotransduction by identifying mechanosensitive ion channels functioning at injurious levels of strain. We detected robust expression of the recently identified mechanosensitive channels, PIEZO1 and PIEZO2. Combined directed expression of Piezo1 and -2 sustained potentiated mechanically induced Ca ²⁺ signals and electrical currents compared with single-Piezo expression. In primary articular chondrocytes, mechanically evoked Ca ²⁺ transients produced by atomic force microscopy were inhibited by GsMTx4, a PIEZO-blocking peptide, and by Piezo1- or Piezo2-specific siRNA. We complemented the cellular approach with an explant-cartilage injury model. GsMTx4 reduced chondrocyte death after mechanical injury, suggesting a possible therapy for reducing cartilage injury and posttraumatic osteoarthritis by attenuating Piezo-mediated cartilage mechanotransduction of injurious strains.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: Animals / Biological Sciences / Biomechanics / Calcium Signaling / Cartilage / Cartilage, Articular - physiology / chondrocytes / Chondrocytes - physiology / cytoskeleton / Electric currents / ion channels / Ion Channels - genetics / Ion Channels - physiology / locomotion / mechanical stress / mechanotransduction / Metabolism / Mice / Osteoarthritis / pathogenesis / Peptides / PNAS Plus / Proteins / RNA, Small Interfering / Strain / Stress, Mechanical / venoms / vertebrates