Journal Article

Trophectoderm biopsy for preimplantation genetic test and technical tips: A review

Kato, Keiichi, Aoyama, Naoki

2020

Overview

Background Recently, the Japan Society of Obstetrics and Gynecology initiated a clinical study of preimplantation genetic test for aneuploidy. There will be a great need for a standardized embryo biopsy technique in Japan. However, the gold standard trophectoderm (TE) biopsy procedure has not been established, and this review outlines the clinical use of TE biopsy. Methods Based on literature, the method and associated techniques for TE biopsy, a dissection method of TE cells from blastocysts, were investigated. Main findings Two TE biopsy methods are used, namely assisted hatching (herniating) and non‐assisted hatching (direct suction); however, it is not clear which of these methods is superior. It is critical to understand whether the flicking or pulling method is beneficial. Conclusion Non‐assisted hatching biopsy method may cause blastocyst collapse with a higher probability, and it may extend the biopsy time. The biopsy procedure should be performed within 3 minutes, and thus direct TE suction may have greater disadvantages. It is a fact that pulling method of TE dissection with laser pulse is simple; however, excess laser shots may induce a higher frequency of mosaicism. It is important to understand that each technique of TE biopsy has benefits and disadvantages.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc,Wiley
• Subject: Aneuploidy / assisted hatching / Biopsy / Births / blastocyst / Blastocysts / Clinical trials / Deoxyribonucleic acid / DNA / Embryonic development / Genetic screening / Gynecology / Hatching / Imprisonment / Laboratories / Mini Review / Mini Reviews / Mosaicism / non‐assisted hatching / Obstetrics / preimplantation genetic testing for aneuploidy / Researchers / Trophectoderm / trophectoderm biopsy