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TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC
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TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC
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Journal Article
TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC
2018
Overview
The mitotic checkpoint ensures accurate chromosome segregation through assembly of the mitotic checkpoint complex (MCC), a soluble inhibitor of the anaphase-promoting complex/cyclosome (APC/C) produced by unattached kinetochores. MCC is also assembled during interphase by Mad1/Mad2 bound at nuclear pores, thereby preventing premature mitotic exit prior to kinetochore maturation and checkpoint activation. Using degron tagging to rapidly deplete the AAA+ ATPase TRIP13, we show that its catalytic activity is required to maintain a pool of open-state Mad2 for MCC assembly, thereby supporting mitotic checkpoint activation, but is also required for timely mitotic exit through catalytic disassembly of MCC. Strikingly, combining TRIP13 depletion with elimination of APC15-dependent Cdc20 ubiquitination/degradation results in a complete inability to exit mitosis, even when MCC assembly at unattached kinetochores is prevented. Thus, mitotic exit requires MCC produced either in interphase or mitosis to be disassembled by TRIP13-catalyzed removal of Mad2 or APC15-driven ubiquitination/degradation of its Cdc20 subunit.
The mitotic checkpoint complex (MCC) is assembled during both mitosis and interphase. Here, the authors use auxin-inducible degron tags to rapidly degrade TRIP13 and find that mitotic exit requires MCC disassembly by TRIP13-catalyzed removal of Mad2 or APC1-driven ubiquitination of Cdc20.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/35
/ 38/32
/ 38/89
/ 82/16
/ 82/29
/ 82/80
/ 82/83
/ 96/106
/ Anaphase
/ Anaphase-Promoting Complex-Cyclosome - genetics
/ Anaphase-Promoting Complex-Cyclosome - metabolism
/ Anaphase-Promoting Complex-Cyclosome - physiology
/ Assembly
/ ATPases Associated with Diverse Cellular Activities - genetics
/ ATPases Associated with Diverse Cellular Activities - metabolism
/ ATPases Associated with Diverse Cellular Activities - physiology
/ Cell Cycle Proteins - genetics
/ Cell Cycle Proteins - metabolism
/ Cell Cycle Proteins - physiology
/ Humanities and Social Sciences
/ M Phase Cell Cycle Checkpoints
/ Mitosis
/ Science
