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Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
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Journal Article
Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
2017
Overview
Cholesterol plays a crucial role in cell membrane organization, dynamics and function. Depletion of cholesterol represents a popular approach to explore cholesterol-sensitivity of membrane proteins. An emerging body of literature shows that the consequence of membrane cholesterol depletion often depends on the
actual
process (acute or chronic), although the molecular mechanism underlying the difference is not clear. Acute depletion, using cyclodextrin-type carriers, is faster relative to chronic depletion, in which inhibitors of cholesterol biosynthesis are used. With the overall goal of addressing molecular differences underlying these processes, we monitored membrane dipole potential under conditions of acute and chronic cholesterol depletion in CHO-K1 cells, using a voltage-sensitive fluorescent dye in dual wavelength ratiometric mode. Our results show that the observed membrane dipole potential exhibits difference under acute and chronic cholesterol depletion conditions,
even when cholesterol content was identical
. To the best of our knowledge, these results provide, for the first time, molecular insight highlighting differences in dipolar reorganization in these processes. A comprehensive understanding of processes in which membrane cholesterol gets modulated would provide novel insight in its interaction with membrane proteins and receptors, thereby allowing us to understand the role of cholesterol in cellular physiology associated with health and disease.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/34
/ 14/63
/ 9/30
/ Animals
/ Humanities and Social Sciences
/ Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology
/ Lipid Metabolism - drug effects
/ Lipid Metabolism Disorders - drug therapy
/ Lipid Metabolism Disorders - etiology
/ Lipid Metabolism Disorders - metabolism
/ Membrane Potentials - drug effects
/ Science
