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Proteomic profiling of the weed feverfew, a neglected pollen allergen source
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Proteomic profiling of the weed feverfew, a neglected pollen allergen source
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Proteomic profiling of the weed feverfew, a neglected pollen allergen source
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Journal Article
Proteomic profiling of the weed feverfew, a neglected pollen allergen source
2017
Overview
Feverfew
(Parthenium hysterophorus)
, an invasive weed from the Asteraceae family, has been reported as allergen source. Despite its relevance, knowledge of allergens is restricted to a partial sequence of a hydroxyproline-rich glycoprotein. We aimed to obtain the entire sequence for recombinant production and characterize feverfew pollen using proteomics and immunological assays. Par h 1, a defensin-proline fusion allergen was obtained by cDNA cloning and recombinantly produced in
E. coli
. Using two complementary proteomic strategies, a total of 258 proteins were identified in feverfew pollen among those 47 proteins belonging to allergenic families. Feverfew sensitized patients’ sera from India revealed IgE reactivity with a pectate lyase, PR-1 protein and thioredoxin in immonoblot. In ELISA, recombinant Par h 1 was recognized by 60 and 40% of Austrian and Indian sera, respectively. Inhibition assays demonstrated the presence of IgE cross-reactive Par h 1, pectate lyase, lipid-transfer protein, profilin and polcalcin in feverfew pollen. This study reveals significant data on the allergenic composition of feverfew pollen and makes recombinant Par h 1 available for cross-reactivity studies. Feverfew might become a global player in weed pollen allergy and inclusion of standardized extracts in routine allergy diagnosis is suggested in exposed populations.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38/90
/ 82/16
/ 82/29
/ 82/51
/ 82/58
/ 82/80
/ 82/83
/ Cloning
/ E coli
/ Enzyme-linked immunosorbent assay
/ Humanities and Social Sciences
/ Immunoglobulin E - immunology
/ Pollen
/ Profilin
/ Proline
/ Proteome
/ Science
/ Tanacetum parthenium - genetics
/ Tanacetum parthenium - immunology
/ Tanacetum parthenium - metabolism
/ Weeds
