Asset Details
Do you wish to reserve the book?
Exosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapy
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Exosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapy
Do you wish to request the book?
Exosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapy
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Exosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapy
2019
Overview
Background
Breast cancer patients under neoadjuvant chemotherapy includes a heterogeneous group of patients who eventually develop distal disease, not detectable by current methods. We propose the use of exosomal miRNAs and circulating tumor cells as diagnostic and predictive biomarkers in these patients.
Methods
Fifty-three breast cancer women initially diagnosed with localized breast cancer under neoadjuvant chemotherapy were prospectively enrolled in this study. However, six of them were later re-evaluated and diagnosed as metastatic breast cancer patients by PET-CT scan. Additionally, eight healthy donors were included. Circulating tumor cells and serum exosomal miRNAs were isolated from blood samples before and at the middle of neoadjuvant therapy and exosomal miRNA levels analyzed by qPCR.
Results
Before neoadjuvant therapy, exosomal miRNA-21 and 105 expression levels were higher in metastatic versus non-metastatic patients and healthy donors. Likewise, higher levels of miRNA-222 were observed in basal-like (
p
= 0.037) and in luminal B versus luminal A (
p
= 0.0145) tumor subtypes. Exosomal miRNA-222 levels correlated with clinical and pathological variables such as progesterone receptor status (
p
= 0.017) and Ki67 (
p
= 0.05). During neoadjuvant treatment, exosomal miRNA-21 expression levels directly correlated with tumor size (
p
= 0.039) and inversely with Ki67 expression (
p
= 0.031). Finally, higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155 were significantly associated with the presence of circulating tumor cells.
Conclusion
Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary clinical tool for improving breast cancer diagnosis and prognosis.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Antineoplastic Agents - therapeutic use
/ Biomarkers, Tumor - genetics
/ Biomarkers, Tumor - isolation & purification
/ Biomedical and Life Sciences
/ Breast Neoplasms - diagnosis
/ EDTA
/ Exosomes
/ Female
/ Gene Expression Profiling - methods
/ Humans
/ MicroRNA
/ MicroRNAs - isolation & purification
/ Neoadjuvant Therapy - methods
/ Neoplastic Cells, Circulating - pathology
/ Oncology
/ Positron Emission Tomography Computed Tomography
/ Surgery
/ Tumors
/ Women
