Journal Article

Systematic integration of molecular and clinical approaches in HCV-induced hepatocellular carcinoma

Yu, Ming-Lung, Shabangu, Ciniso Sylvester, Su, Wen-Hsiu, Wang, Shu-Chi, Dai, Chia-Yen, Huang, Jee-Fu, Li, Chia-Yang, Chuang, Wan-Long

2024

Overview

Background MicroRNAs (miRNAs) play a crucial role in gene expression and regulation, with dysregulation of miRNA function linked to various diseases, including hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC). There is still a gap in understanding the regulatory relationship between miRNAs and mRNAs in HCV-HCC. This study aimed to investigate the function and effects of persistent HCV-induced miRNA expression on gene regulation in HCC. Methods MiRNA array data were used to identify differentially expressed miRNAs and their targets, and miRNAs were analyzed via DIANA for KEGG pathways, gene ontology (GO) functional enrichment, and Ingenuity Pathways Analysis (IPA) for hepatotoxicity, canonical pathways, associated network functions, and interactive networks. Results Seventeen miRNAs in L-HCV and 9 miRNAs in S-HCV were differentially expressed, and 5 miRNAs in L-HCV and 5 miRNAs in S-HCV were significantly expressed in liver hepatocellular carcinoma (LIHC) tumors. Grouped miRNA survival analysis showed that L-HCV miRNAs were associated with survival in LIHC, and miRNA‒mRNA targets regulated viral carcinogenesis and cell cycle alteration through cancer pathways in LIHC. MiRNA-regulated RCN1 was suppressed through miRNA-oncogene interactions, and suppression of RCN1 inhibited invasion and migration in HCC. Conclusion Persistent HCV infection induced the expression of miRNAs that act as tumor suppressors by inhibiting oncogenes in HCC. RCN1 was suppressed while miRNAs were upregulated, demonstrating an inverse relationship. Therefore, hsa-miR-215-5p, hsa-miR-10b-5p, hsa-let-7a-5p and their target RCN1 may be ideal biomarkers for monitoring HCV-HCC progression. Graphical Abstract

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Antibodies / Antigens / Antiviral agents / Biomarkers / Biomedical and Life Sciences / Biomedicine / Carcinogenesis / Care and treatment / Cell cycle / Control / Development and progression / Diagnosis / Dosage and administration / Gene expression / Gene regulation / Genes / Genomes / HCC / HCV / Health aspects / Health maintenance organizations / Hepatitis C / Hepatitis C virus / Hepatocellular carcinoma / Hepatoma / Hepatotoxicity / Identification and classification / ImmunoVirology and ImmunoOncology / Infections / International economic relations / Liver cancer / Liver cirrhosis / Medicine/Public Health / Messenger RNA / MicroRNA / MicroRNAs / miRNA / Oncogenes / Patients / RCN1 / Risk factors / Scientific equipment and supplies industry / Software / Viral infections