Journal Article

Recombinant mannan-binding lectin magnetic beads increase pathogen detection in immunocompromised patients

Ru-Li, Feng, Yu-Jun, Dong, Hao, Zheng, Jin-Xing, Lu, Xiao-Ping, Chen, Ze-Yin, Liang

2024

Overview

The microbiological diagnosis of infection for hematological malignancy patients receiving chemotherapy or allogeneic hematopoietic stem cell transplantation (allo-HSCT) patients relies primarily on standard microbial culture, especially blood culture, which has many shortcomings, such as having low positive rates, being time-consuming and having a limited pathogenic spectrum. In this prospective observational self-controlled test accuracy study, blood, cerebrospinal fluid (CSF), and bronchoalveolar lavage fluid (BALF) samples were collected from chemotherapy or allo-HSCT patients with clinical symptoms of infections who were hospitalized at Peking University First Hospital. Possible pathogens were detected by the method based on recombinant mannan-binding lectin (MBL) magnetic bead enrichment (M1 method) and simultaneously by a standard method. The analytical sensitivity of M1 method was close to that of standard culture method. Besides, the turn-around time of M1-method was significantly shorter than that of standard culture method. Moreover, the M1 method also added diagnostic value through the detection of some clinically relevant microbes missed by the standard method. M1 method could significantly increase the detection efficiency of pathogens (including bacteria and fungi) in immunocompromised patients. Key points • The detection results of M1-method had a high coincidence rate with that of standard method • M1 method detected many pathogens which had not been found by standard clinic method

• Publisher: Springer Berlin Heidelberg,Springer,Springer Nature B.V
• Subject: Allografts / Analysis / Bacteria / Binding / Biomedical and Life Sciences / Biotechnology / Blood / Blood culture / Bronchoalveolar Lavage Fluid / Bronchus / Cell culture / cell transplantation / Cerebrospinal fluid / Chemotherapy / detection limit / disease diagnosis / drug therapy / Hematopoietic stem cells / High-Throughput Nucleotide Sequencing / hospitals / Humans / Identification and classification / Immunocompromised Host / Immunocompromised hosts / Lavage / lectins / Life Sciences / Magnetic Phenomena / magnetism / Malignancy / Mannan / Mannan-binding lectin / Mannose-Binding Lectin / Methods and Protocols / microbial culture / microbial detection / Microbial Genetics and Genomics / Microbiology / Microorganisms / Pathogens / Patients / Stem cell transplantation / Stem cells / Transplantation