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A CREB3–ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens
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A CREB3–ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens
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Journal Article
A CREB3–ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens
2013
Overview
Treatment of cells with brefeldin A (BFA) blocks secretory vesicle transport and causes a collapse of the Golgi apparatus. To gain more insight into the cellular mechanisms mediating BFA toxicity, we conducted a genome-wide haploid genetic screen that led to the identification of the small G protein ADP-ribosylation factor 4 (ARF4). ARF4 depletion preserves viability, Golgi integrity and cargo trafficking in the presence of BFA, and these effects depend on the guanine nucleotide exchange factor GBF1 and other ARF isoforms including ARF1 and ARF5. ARF4 knockdown cells show increased resistance to several human pathogens including
Chlamydia trachomatis
and
Shigella flexneri
. Furthermore, ARF4 expression is induced when cells are exposed to several Golgi-disturbing agents and requires the CREB3 (also known as Luman or LZIP) transcription factor, whose downregulation mimics ARF4 loss. Thus, we have uncovered a CREB3–ARF4 signalling cascade that may be part of a Golgi stress response set in motion by stimuli compromising Golgi capacity.
In an insertional mutagenesis screen, Sabatini and colleagues identify the small G protein ARF4 as a mediator of cell death in response to brefeldin A (BFA) treatment. BFA-induced Golgi stress upregulates ARF4, and loss of ARF protects against propagation of pathogens known to induce Golgi fragmentation.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ ADP-Ribosylation Factor 1 - genetics
/ ADP-Ribosylation Factor 1 - metabolism
/ ADP-Ribosylation Factors - genetics
/ ADP-Ribosylation Factors - metabolism
/ Biology
/ Cellular signal transduction
/ Chlamydia trachomatis - physiology
/ Cyclic AMP Response Element-Binding Protein - metabolism
/ Genomes
/ Golgi Apparatus - drug effects
/ Golgi Apparatus - physiology
/ Guanine Nucleotide Exchange Factors - genetics
/ Guanine Nucleotide Exchange Factors - metabolism
/ Humans
/ Identification and classification
/ Protein Transport - drug effects
/ Proteins
/ RNA, Small Interfering - genetics
/ Shigella flexneri - physiology
/ Toxicity
