Asset Details
Do you wish to reserve the book?
Validation of reference genes for real-time quantitative PCR studies in gene expression levels of Lactobacillus casei Zhang
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Validation of reference genes for real-time quantitative PCR studies in gene expression levels of Lactobacillus casei Zhang
Do you wish to request the book?
Validation of reference genes for real-time quantitative PCR studies in gene expression levels of Lactobacillus casei Zhang
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Validation of reference genes for real-time quantitative PCR studies in gene expression levels of Lactobacillus casei Zhang
2011
Overview
Abstract
Lactobacillus casei Zhang, a potential probiotic strain isolated from homemade koumiss in Inner Mongolia of China, has been sequenced and deposited in GenBank. Real-time quantitative PCR is one of the most widely used methods to study related gene expression levels of Lactobacillus casei Zhang. For accurate and reliable gene expression analysis, normalization of gene expression data using one or more appropriate reference genes is essential. We used three statistical methods (geNorm, NormFinder, and BestKeeper) to evaluate the expression levels of five candidate reference genes (GAPD, gyrB, LDH, 16s rRNA, and recA) under different culture conditions and different growth phases to find a suitable housekeeping gene which can be used as internal standard. The results showed that the best reference gene was GAPD, and a set of two genes, GAPD and gyrB (which were the most stable reference genes), is recommended for normalization of real-time quantitative PCR experiments under all the different experimental conditions tested. The systematic validation of candidate reference genes is important for obtaining reliable analysis results of real-time quantitative PCR studies in gene expression levels of Lactobacillus casei Zhang.
Publisher
Oxford University Press,Springer-Verlag,Springer
Subject
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ China
/ Fundamental and applied biological sciences. Psychology
/ genes
/ genetics
/ Lactobacillus casei - genetics
/ Lactobacillus casei - metabolism
/ Nitrogen
/ Real-Time Polymerase Chain Reaction
/ Real-Time Polymerase Chain Reaction - standards
/ Studies
