Journal Article

Exosome Loaded in Microneedle Patch Ameliorates Renal Ischemia–Reperfusion Injury in a Mouse Model

Abedanzadeh, Mozhgan, Heidari, Reza, Aghdaei, Mahdokht Hossein, Azarpira, Negar, Hashemi, Mehrdad, Sabegh, Alireza, Keshtkar, Somayeh, Taghavi, Samin, Dara, Mahintaj, Abolmaali, Samira Sadat

2025

Overview

Introduction: Renal dysfunction due to ischemia–reperfusion injury (IRI) is a common problem after kidney transplantation. In recent years, studies on animal models have shown that exosomes derived from mesenchymal stem cells (MSC-Exo) play an important role in treating acute kidney injury (AKI) and promoting tissue repair. The microneedle patch provides a noninvasive and targeted delivery system for exosomes. The purpose of this innovative approach is to combine MSC-Exo with microneedle patches.Method: Exosomes were isolated from MSCs, characterized, and placed in the prepared microneedle patch. Then this construct was applied to the IRI mice model. After 7 days, the gene expression of miR-34a and its targets B-cell lymphoma-2 (BCL-2) and BCL-2-associated X (BAX), along with reactive oxygen species (ROS) and lipid peroxidation (LPO) production, was investigated. Additionally, renoprotection was evaluated for measuring blood urea nitrogen (BUN) and creatinine (Cr) and histopathology detection.Results: After using microneedle patches containing exosomes, the reduction of miR-34a and BAX and enhancement of BCL-2 were observed. Moreover, treatment by this construct decreased the production of ROS, LPO, BUN, and Cr and improved tissue damage.Conclusion: The use of a microneedle patch containing exosomes is a noninvasive method that enables the release of exosomes in a slow manner. In comparison to exosome injection alone, microneedle patch-exosome treatment offers a longer and more targeted effect that improves renal IRI dysfunction and reduces tissue damage, potentially facilitating the clinical application of exosomes and improving graft survival.

• Publisher: Wiley,John Wiley & Sons, Inc
• Subject: Analysis / Animal models / Apoptosis / Aqueous solutions / BAX protein / Bcl-2 protein / Cell culture / Creatinine / Damage / Ethylenediaminetetraacetic acid / Exosomes / Gene expression / Genes / Histopathology / Hydrogels / Injuries / Investigations / Ischemia / Kidney transplantation / Kidneys / Laboratory animals / Lipid peroxidation / Lipids / Lymphocytes B / Lymphoma / Mesenchymal stem cells / MicroRNAs / Needles / Peroxidation / Polyvinyl alcohol / Reactive oxygen species / Reagents / Renal function / Reperfusion / Reperfusion injury / Stem cell transplantation / Stem cells / Transplantation