Asset Details
Do you wish to reserve the book?
Recellularization of Rat Liver Scaffolds by Human Liver Stem Cells
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Recellularization of Rat Liver Scaffolds by Human Liver Stem Cells
Do you wish to request the book?
Recellularization of Rat Liver Scaffolds by Human Liver Stem Cells
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Recellularization of Rat Liver Scaffolds by Human Liver Stem Cells
2015
Overview
In the present study, rat liver acellular scaffolds were used as biological support to guide the differentiation of human liver stem-like cells (HLSC) to hepatocytes. Once recellularized, the scaffolds were maintained for 21 days in different culture conditions to evaluate hepatocyte differentiation. HLSC lost the embryonic markers (alpha-fetoprotein, nestin, nanog, sox2, Musashi1, Oct 3/4, and pax2), increased the expression of albumin, and acquired the expression of lactate dehydrogenase and three subtypes of cytochrome P450. The presence of urea nitrogen in the culture medium confirmed their metabolic activity. In addition, cells attached to tubular remnant matrix structures expressed cytokeratin 19, CD31, and vimentin. The rat extracellular matrix (ECM) provides not only a favorable environment for differentiation of HLSC in functional hepatocytes (hepatocyte like) but also promoted the generation of some epithelial-like and endothelial-like cells. When fibroblast growth factor–epidermal growth factor or HLSC-derived conditioned medium was added to the perfusate, an improvement of survival rate was observed. The conditioned medium from HLSC potentiated also the metabolic activity of hepatocyte-like cells repopulating the acellular liver. In conclusion, HLSC have the potential, in association with the natural ECM, to generate
in vitro
a functional “humanized liver-like tissue.”
Publisher
Mary Ann Liebert, Inc,SAGE Publications
Subject
/ Animals
/ Culture Media, Conditioned - chemistry
/ Cytochrome P-450 Enzyme System - biosynthesis
/ Cytochrome P-450 Enzyme System - genetics
/ Fetal Proteins - biosynthesis
/ Humans
/ Intermediate Filament Proteins - biosynthesis
/ Intermediate Filament Proteins - genetics
/ L-Lactate Dehydrogenase - biosynthesis
/ L-Lactate Dehydrogenase - genetics
/ Liver
/ Male
/ Original
/ Platelet Endothelial Cell Adhesion Molecule-1 - biosynthesis
/ Platelet Endothelial Cell Adhesion Molecule-1 - genetics
/ Rats
/ Rodents
