Asset Details

MbrlCatalogueTitleDetail

Do you wish to reserve the book?

A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

by Bush, Elizabeth , Nita, Akiko , Oliver, Charlotte , Jones, Taylor , Kawaguchi, Akira , McHenry, Diana , Katori, Miyuki , Nita, Mizuho

in ambient temperature / Blotch / Diseases and pests / Extraction (Chemistry) / Genetic aspects / GLRaV2 / GLRaV3 / Grapes / Grapevine leafroll-associated virus 2 / Grapevine red blotch virus / Grapevine rupestris stem pitting-associated virus / Grapevine virus A / Grapevine virus B / grapevine viruses / GRBV / Health aspects / Identification and classification / Management / Membranes / Membranes (Technology) / Methods / NitroPure nitrocellulose / nucleic acids / Plant viruses / Sampling / sap / Stem pitting / Surveys / Vineyards / Virus diseases of plants / Viruses / Vitis / Wineries & vineyards

2023

Yes Please

Hey, we have placed the reservation for you!

By the way, why not check out events that you can attend while you pick your title.

Oops! Something went wrong.

Looks like we were not able to place the reservation. Kindly try again later.

Are you sure you want to remove the book from the shelf?

A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

by Bush, Elizabeth , Nita, Akiko , Oliver, Charlotte , Jones, Taylor , Kawaguchi, Akira , McHenry, Diana , Katori, Miyuki , Nita, Mizuho

2023

Confirm

Do you wish to request the book?

A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

by Bush, Elizabeth , Nita, Akiko , Oliver, Charlotte , Jones, Taylor , Kawaguchi, Akira , McHenry, Diana , Katori, Miyuki , Nita, Mizuho

2023

Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy

How would you like to get it?

Submit

We have requested the book for you!

Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.

Oops! Something went wrong.

Looks like we were not able to place your request. Kindly try again later.

Journal Article

A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

Bush, Elizabeth,

Nita, Akiko,

Oliver, Charlotte,

Jones, Taylor,

Kawaguchi, Akira,

McHenry, Diana,

Katori, Miyuki,

Nita, Mizuho

2023

Overview

We developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap for grapevine virus detection. We devised an efficient nucleic acid extraction method for the NPN membrane, resulting in 100% amplification success for grapevine leafroll-associated virus 2 (GLRaV2) and 3 (GLRaV3), grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine virus A, grapevine virus B, and grapevine red blotch virus (GRBV). This method also allowed the storage of recoverable nucleic acid for 18 months at room temperature. We created a sampling kit to survey GLRaV2, GLRaV3, and GRBV in Japanese vineyards. We tested the kits in the field in 2018 and then conducted mail-in surveys in 2020–2021. The results showed a substantial prevalence of GLRaV3, with 48.5% of 132 sampled vines being positive. On the other hand, only 3% of samples tested positive for GLRaV2 and none for GRBV.

Share this book

Add to My Shelf

Publisher

MDPI AG,MDPI

Subject

ambient temperature

/ Blotch

/ Diseases and pests

/ Extraction (Chemistry)

/ Genetic aspects

/ GLRaV2

/ GLRaV3