Journal Article

Foot and Mouth Disease Vaccine Matching and Post-Vaccination Assessment in Abu Dhabi, United Arab Emirates

Khan, Rashid A., Kayaf, Kaltham, Wood, Britta A., Ishag, Hassan Zackaria Ali, Eltahir, Yassir M., Parekh, Krupali, Bensalah, Oum Keltoum, Mohamed, Meera Saeed, King, Donald P., Ludi, Anna, Shah, Asma Abdi Mohamed

2024

Overview

Despite the annual vaccination of livestock against foot and mouth disease (FMD) in the United Arab Emirates (UAE), outbreaks of the disease continue to be reported. The effective control of field outbreaks by vaccination requires that the vaccines used are antigenically matched to circulating field FMD viruses. In this study, a vaccine matching analysis was performed using the two-dimensional virus neutralization test (VNT) for three field isolates belonging to the O/ME-SA/PanAsia-2/ANT-10 and O/ME-SA/SA-2018 lineages collected from different FMD outbreaks that occurred within the Abu Dhabi Emirate in 2021 affecting Arabian oryx (Oryx leucoryx), goat, and sheep. In addition, post-vaccination antibodies in sheep and goats were measured using solid-phase competitive ELISA (SPCE) for FMDV serotypes A and O at five months after a single vaccine dose and a further 28 days later after a second dose of the FMD vaccine. An analysis of vaccine matching revealed that five out of the six vaccine strains tested were antigenically matched to the UAE field isolates, with r1-values ranging between 0.32 and 0.75. These results suggest that the vaccine strains (O-3039 and O1 Manisa) included in the FMD vaccine used in the Abu Dhabi Emirate are likely to provide protection against outbreaks caused by the circulating O/ME-SA/PanAsia-2/ANT-10 and O/ME-SA/SA-2018 lineages. All critical residues at site 1 and site 3 of VP1 were conserved in all isolates, although an analysis of the VP1-encoding sequences revealed 14–16 amino acid substitutions compared to the sequence of the O1 Manisa vaccine strain. This study also reports on the results of post-vaccination monitoring where the immunization coverage rates against FMDV serotypes A and O were 47% and 69% five months after the first dose of the FMD vaccine, and they were increased to 81 and 88%, respectively, 28 days after the second dose of the vaccine. These results reinforce the importance of using a second booster dose to maximize the impact of vaccination. In conclusion, the vaccine strains currently used in Abu Dhabi are antigenically matched to circulating field isolates from two serotype O clades (O/ME-SA/PanAsia-2/ANT-10 sublineage and O/ME-SA/SA-2018 lineage). The bi-annual vaccination schedule for FMD in the Abu Dhabi Emirate has the potential to establish a sufficient herd immunity, especially when complemented by additional biosecurity measures for comprehensive FMD control. These findings are pivotal for the successful implementation of the region’s vaccination-based FMD control policy, showing that high vaccination coverage and the wide-spread use of booster doses in susceptible herds is required to achieve a high level of FMDV-specific antibodies in vaccinated animals.

• Publisher: MDPI AG,MDPI
• Subject: Amino acid sequence / amino acid substitution / Amino acids / Analysis / Animals / annuals / Antibodies / biosecurity / Cattle / Conserved sequence / disease outbreaks / Disease transmission / dosage / Enzyme-linked immunosorbent assay / Epidemics / Farms / fields / Foot & mouth disease / Foot-and-mouth disease / Foot-and-mouth disease virus / Goats / Herd immunity / herding / herds / International economic relations / issues and policy / Laboratories / Livestock / Medical research / Medicine, Experimental / monitoring / neutralization tests / Oryx leucoryx / Outbreaks / Pharmaceutical industry / post-vaccination assessment / sequence analysis / Serotypes / Sheep / Strains (organisms) / testing / United Arab Emirates / Vaccination / vaccine matching / Vaccines / Viruses / VP1 protein