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Quantitative 3D Video Microscopy of HIV Transfer Across T Cell Virological Synapses
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Quantitative 3D Video Microscopy of HIV Transfer Across T Cell Virological Synapses
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Journal Article
Quantitative 3D Video Microscopy of HIV Transfer Across T Cell Virological Synapses
2009
Overview
The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized \"buttons\" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
Biological and medical sciences
/ CD4-Positive T-Lymphocytes - physiology
/ CD4-Positive T-Lymphocytes - ultrastructure
/ CD4-Positive T-Lymphocytes - virology
/ Cytochalasin D - pharmacology
/ gag Gene Products, Human Immunodeficiency Virus - metabolism
/ HIV
/ Human immunodeficiency virus
/ Humans
/ Imaging
/ Immunodeficiencies. Immunoglobulinopathies
/ Microscopy, Electron, Transmission
/ Receptors, CCR5 - metabolism
/ Receptors, CXCR4 - metabolism
/ Recombinant Fusion Proteins - metabolism
/ synapse
/ Synapses
/ Viral diseases of the lymphoid tissue and the blood. Aids
/ Virology
/ Viruses
